Bogø Jensen Lars, Willems Rob J L, van den Bogaard A E
Section for Antimicrobial Resistance and Environmental Microbiology, Danish Veterinary Laboratory, Copenhagen, Denmark.
APMIS. 2003 Jun;111(6):669-72. doi: 10.1034/j.1600-0463.2003.1110610.x.
Glycopeptide resistant enterococci (GRE) isolated from animals and humans were characterised using both AFPL typing and genetic characterisation of the glycopeptide resistance transposon Tn1546. All isolates were collected in 1997 when the glycopeptide avoparcin was still being used as growth promoter. All investigated animal isolates were from mixed pig and poultry farms in the Netherlands and the human isolated from the farmers of these farms. A total of 24 isolates were investigated. AFLP and Tn1546 typing revealed that both pig and poultry related enterococcal and vanA transposon genotypes were found among the human isolates indicating spread of glycopeptide resistance from both pig and poultry to the farmers. These findings contradict previous finding that showed that GRE recovered from the general population were genotypically undistinguishable from GRE isolated from pigs but are in line with other studies that demonstrated spread of GRE from poultry to farmers in poultry farms.
采用扩增片段长度多态性（AFPL）分型和糖肽抗性转座子Tn1546的基因特征分析，对从动物和人类中分离出的耐糖肽肠球菌（GRE）进行了表征。所有分离株均采集于1997年，当时糖肽阿伏帕星仍被用作生长促进剂。所有调查的动物分离株均来自荷兰的混合猪场和家禽场，人类分离株则来自这些农场的农民。共调查了24株分离株。AFPL和Tn1546分型显示，在人类分离株中发现了与猪和家禽相关的肠球菌及vanA转座子基因型，这表明糖肽抗性从猪和家禽传播到了农民。这些发现与之前的研究结果相矛盾，之前的研究表明，从普通人群中分离出的GRE在基因型上与从猪中分离出的GRE无法区分，但与其他研究结果一致，其他研究表明GRE在家禽场中从家禽传播到了农民。
