Montes M, Farto R, Pérez M J, Nieto T P, Larsen J L, Christensen H
Departmento de Biología Funcional y Ciencias de la Salud, Facultad de Ciencias Universidad de Vigo, Vigo, Spain.
J Appl Microbiol. 2003;95(4):693-703. doi: 10.1046/j.1365-2672.2003.02028.x.
The aim of the present study was to clarify the taxonomic status of Vibrio strains isolated from an aquaculture system and to compare the results of the identifications made by phenotypic and molecular methods.
Fifty-one Vibrio strains isolated from a turbot (Scophthalmus maximus) aquaculture system were characterized by ribotyping and 16S rRNA gene sequencing. The strains had been identified phenotypically in a previous numerical taxonomy analysis as Vibrio anguillarum, V. mediterranei, V. splendidus, V. aestuarianus, V. ordalii, V. fischeri and V. scophthalmi. Cluster analysis of ribotype patterns showed that the strains were separated into two main groups: V. splendidus-V. lentus and V. scophthalmi groups. The use of 16S rRNA gene sequence allowed differentiation among V. splendidus biovar I and V. lentus strains.
The molecular methods identified strains of V. splendidus biovar I, V. lentus and V. scophthalmi, showing discrepancies with phenotypic characterization.
The molecular methods, as 16S rRNA gene sequence analysis, are necessary for the identification of phenotypically close species to avoid mis-identifications. Interestingly, this is the first report of V. lentus strains associated to turbot culture.
本研究旨在阐明从水产养殖系统中分离出的弧菌菌株的分类地位，并比较通过表型和分子方法进行鉴定的结果。
从大菱鲆（Scophthalmus maximus）水产养殖系统中分离出的51株弧菌菌株通过核糖体分型和16S rRNA基因测序进行了特征分析。这些菌株在先前的数值分类分析中已通过表型鉴定为鳗弧菌、地中海弧菌、灿烂弧菌、河口弧菌、奥氏弧菌、费氏弧菌和大菱鲆弧菌。核糖体分型模式的聚类分析表明，这些菌株被分为两个主要组：灿烂弧菌-迟缓弧菌组和大菱鲆弧菌组。16S rRNA基因序列的使用允许区分灿烂弧菌生物变种I和迟缓弧菌菌株。
分子方法鉴定出了灿烂弧菌生物变种I、迟缓弧菌和大菱鲆弧菌的菌株，显示出与表型特征的差异。
分子方法，如16S rRNA基因序列分析，对于鉴定表型相似的物种以避免错误鉴定是必要的。有趣的是，这是关于迟缓弧菌菌株与大菱鲆养殖相关的首次报道。
