维泰克系统将维氏气单胞菌温和生物变种误鉴定为溶藻弧菌。

Misidentification of Aeromonas veronii biovar sobria as Vibrio alginolyticus by the Vitek system.

作者信息

Park T S, Oh S H, Lee E Y, Lee T K, Park K H, Figueras M J, Chang C L

机构信息

Department of Laboratory Medicine and Internal Medicine, College of Medicine, Pusan National University, Busan, Korea.

出版信息

Lett Appl Microbiol. 2003;37(4):349-53. doi: 10.1046/j.1472-765x.2003.01410.x.

DOI:10.1046/j.1472-765x.2003.01410.x

PMID:12969502

Abstract

AIMS

To find the cause of misidentification of aeromonads when using the Vitek system.

METHODS AND RESULTS

Two Aeromonas veronii biovar sobria isolates were misidentified as Vibrio alginolyticus by the Vitek system. Both strains' identification was confirmed by biochemical testing, API 20E/20NE kits and/or 16S RFLP analysis. Thirty-one known Aeromonas species were tested by the Vitek system using 0.45 and 0.85% saline in the suspension medium. It was not clear whether low salinity causes misidentification of Aeromonas species more frequently.

CONCLUSIONS

The specified reaction time may be inappropriately short for some critical biochemical tests of some strains. An ingenious reading strategy regarding incubation time is necessary to improve identification of Aeromonas species by the Vitek system.

SIGNIFICANCE AND IMPACT OF THE STUDY

To our knowledge, this is the first report of misidentification of A. veronii biovar sobria as V. alginolyticus in the Vitek system.

摘要

目的

找出使用Vitek系统时气单胞菌鉴定错误的原因。

方法与结果

两株维氏气单胞菌温和生物变种分离株被Vitek系统误鉴定为溶藻弧菌。两株菌的鉴定通过生化试验、API 20E/20NE试剂盒和/或16S RFLP分析得以确认。使用0.45%和0.85%的生理盐水作为悬浮培养基，通过Vitek系统对31种已知气单胞菌进行了检测。尚不清楚低盐度是否更频繁地导致气单胞菌属的鉴定错误。