评估两种小型系统，即MicroScan W/A和BBL Crystal E/NF，用于鉴定气单胞菌属临床分离株。
Evaluation of two miniaturized systems, MicroScan W/A and BBL Crystal E/NF, for identification of clinical isolates of Aeromonas spp.
作者信息
Soler L, Marco F, Vila J, Chacón M R, Guarro J, Figueras M J
机构信息
Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, 43201 Reus, Spain.
出版信息
J Clin Microbiol. 2003 Dec;41(12):5732-4. doi: 10.1128/JCM.41.12.5732-5734.2003.
Abstract
Fifty-two clinical strains and 22 type and reference Aeromonas strains, previously genetically characterized by 16S rRNA gene restriction fragment length polymorphism, were identified in parallel with the MicroScan Walk/Away and BBL Crystal Enteric/Nonfermenter systems. The former identified only 14.8% of the isolates correctly, and the latter identified only 20.3% correctly, which indicates that neither of these systems is useful for this purpose.
摘要
通过16S rRNA基因限制性片段长度多态性先前已进行基因特征分析的52株临床菌株以及22株嗜水气单胞菌标准菌株和参考菌株，采用MicroScan Walk/Away和BBL Crystal肠道/非发酵菌鉴定系统进行平行鉴定。前者仅正确鉴定出14.8%的分离株，后者仅正确鉴定出20.3%，这表明这两种系统均不适用于此目的。
相似文献
1
Evaluation of two miniaturized systems, MicroScan W/A and BBL Crystal E/NF, for identification of clinical isolates of Aeromonas spp.评估两种小型系统，即MicroScan W/A和BBL Crystal E/NF，用于鉴定气单胞菌属临床分离株。
J Clin Microbiol. 2003 Dec;41(12):5732-4. doi: 10.1128/JCM.41.12.5732-5734.2003.
2
Identification of Aeromonas culicicola by 16S rDNA RFLP.通过16S rDNA限制性片段长度多态性分析鉴定库蚊气单胞菌
Pol J Microbiol. 2005;54(4):335-8.
3
Rapid identification of Aeromonas species using 16S rDNA targeted oligonucleotide primers: a molecular approach based on screening of environmental isolates.使用16S rDNA靶向寡核苷酸引物快速鉴定气单胞菌属菌种：一种基于环境分离株筛选的分子方法。
J Appl Bacteriol. 1994 Dec;77(6):722-6. doi: 10.1111/j.1365-2672.1994.tb02825.x.
4
Species identification of clinically important Aeromonas spp. by restriction fragment length polymorphism of 16S rDNA.通过16S rDNA的限制性片段长度多态性对临床重要气单胞菌属进行菌种鉴定。
Lett Appl Microbiol. 2007 May;44(5):550-4. doi: 10.1111/j.1472-765X.2006.02104.x.
5
Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes.通过PCR扩增的16S rRNA基因的限制性片段长度多态性鉴定气单胞菌临床分离株。
J Clin Microbiol. 1997 Jul;35(7):1671-4. doi: 10.1128/jcm.35.7.1671-1674.1997.
6
rRNA gene restriction patterns as taxonomic tools for the genus Aeromonas.核糖体RNA基因限制性图谱作为气单胞菌属的分类工具。
Int J Syst Bacteriol. 1992 Jul;42(3):384-9. doi: 10.1099/00207713-42-3-384.
7
Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish.比较从患病鱼类中分离的气单胞菌菌株的表型和遗传鉴定。
Syst Appl Microbiol. 2010 Apr;33(3):149-53. doi: 10.1016/j.syapm.2010.02.002. Epub 2010 Mar 15.
8
Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas.利用16S rDNA和gyrB基因的变性梯度凝胶电泳（DGGE）技术对气单胞菌属成员进行分型的评估。
FEMS Microbiol Lett. 2005 May 1;246(1):11-8. doi: 10.1016/j.femsle.2005.03.033.
9
On the identification of clinical Aeromonas by a new restriction fragment length polymorphism of 16S rDNA method.关于通过16S rDNA方法的一种新的限制性片段长度多态性鉴定临床气单胞菌。
Lett Appl Microbiol. 2007 Dec;45(6):692-3; author reply 694-5. doi: 10.1111/j.1472-765X.2007.02245.x. Epub 2007 Oct 4.
10
Phylogenetic evidence suggests that strains of Aeromonas hydrophila subsp. dhakensis belong to the species Aeromonas aquariorum sp. nov.系统发育学证据表明，嗜水气单胞菌达卡亚种菌株属于新种水族气单胞菌。
Curr Microbiol. 2009 Jan;58(1):76-80. doi: 10.1007/s00284-008-9278-6. Epub 2008 Oct 7.
引用本文的文献
1
Technical specifications for a EU-wide baseline survey of antimicrobial resistance in bacteria from aquaculture animals.欧盟范围内水产养殖动物细菌耐药性基线调查的技术规范
EFSA J. 2024 Jul 31;22(7):e8928. doi: 10.2903/j.efsa.2024.8928. eCollection 2024 Jul.
2
Aeromonas species obtained from different farmed aquatic species in India and Taiwan show high phenotypic relatedness despite species diversity.印度和台湾从不同养殖水生物种中获得的气单胞菌属表现出高表型相关性，尽管物种多样性很高。
BMC Res Notes. 2021 Aug 16;14(1):313. doi: 10.1186/s13104-021-05716-3.
3
Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Complex Isolated from .基于分子分型、抗菌谱和聚合酶链反应-限制性片段长度多态性的从……分离出的复合体检测
Pathogens. 2020 Mar 22;9(3):238. doi: 10.3390/pathogens9030238.
4
An Update on the Genus : Taxonomy, Epidemiology, and Pathogenicity.属的最新情况：分类学、流行病学及致病性
Microorganisms. 2020 Jan 17;8(1):129. doi: 10.3390/microorganisms8010129.
5
Reassessment of the Enteropathogenicity of Mesophilic Species.嗜温菌属肠道致病性的重新评估
Front Microbiol. 2016 Sep 21;7:1395. doi: 10.3389/fmicb.2016.01395. eCollection 2016.
6
Molecular characterization of clinical isolates of Aeromonas species from Malaysia.马来西亚气单胞菌临床分离株的分子特征。
PLoS One. 2012;7(2):e30205. doi: 10.1371/journal.pone.0030205. Epub 2012 Feb 27.
7
Identification and genomovar assignation of clinical strains of Pseudomonas stutzeri.鉴定和基因组变异群指派临床菌株恶臭假单胞菌。
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2133-9. doi: 10.1007/s10096-012-1547-4. Epub 2012 Jan 27.
8
The genus Aeromonas: taxonomy, pathogenicity, and infection.气单胞菌属：分类、致病性和感染。
Clin Microbiol Rev. 2010 Jan;23(1):35-73. doi: 10.1128/CMR.00039-09.
9
Clinical relevance of the recently described species Aeromonas aquariorum.最近描述的物种嗜水气单胞菌的临床相关性。
J Clin Microbiol. 2009 Nov;47(11):3742-6. doi: 10.1128/JCM.02216-08. Epub 2009 Sep 9.
10
Comparative evaluation of automated reading versus visual interpretation with the BBL Crystal enteric/nonfermenter identification system in a clinical setting.在临床环境中使用BBL Crystal肠道/非发酵菌鉴定系统对自动读数与目视判读进行比较评估。
Eur J Clin Microbiol Infect Dis. 2007 Jun;26(6):443-4. doi: 10.1007/s10096-007-0317-1.
本文引用的文献
1
Misidentification of Aeromonas veronii biovar sobria as Vibrio alginolyticus by the Vitek system.维泰克系统将维氏气单胞菌温和生物变种误鉴定为溶藻弧菌。
Lett Appl Microbiol. 2003;37(4):349-53. doi: 10.1046/j.1472-765x.2003.01410.x.
2
Aeromonas infection of the hepatobiliary system: report of 15 cases and review of the literature.气单胞菌属引起的肝胆系统感染：15例报告及文献复习
Clin Infect Dis. 2003 Aug 15;37(4):506-13. doi: 10.1086/376629. Epub 2003 Jul 30.
3
The genus Aeromonas: biochemical characteristics, atypical reactions, and phenotypic identification schemes.气单胞菌属：生化特性、非典型反应及表型鉴定方法
J Clin Microbiol. 2003 Jun;41(6):2348-57. doi: 10.1128/JCM.41.6.2348-2357.2003.
4
Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico.从墨西哥供人类食用的冷冻鱼中分离出的气单胞菌属细菌的特性研究。
Int J Food Microbiol. 2003 Jul 15;84(1):41-9. doi: 10.1016/s0168-1605(02)00393-8.
5
A DNA probe specific for Aeromonas colonies.一种针对气单胞菌菌落的DNA探针。
Diagn Microbiol Infect Dis. 2002 Nov;44(3):221-5. doi: 10.1016/s0732-8893(02)00455-8.
6
Phenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp (Cyprinus carpio L.).从鲤鱼（Cyprinus carpio L.）分离的气单胞菌基因组种的表型特征和致病性
J Appl Microbiol. 2002;93(6):1034-41. doi: 10.1046/j.1365-2672.2002.01784.x.
7
Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis.通过16S rDNA RFLP分析鉴别气单胞菌属的扩展方法。
Int J Syst Evol Microbiol. 2000 Nov;50 Pt 6:2069-2073. doi: 10.1099/00207713-50-6-2069.
8
Clinically relevant Aeromonas species.具有临床相关性的气单胞菌属菌种。
Clin Infect Dis. 2000 Jun;30(6):988-9. doi: 10.1086/313839.
9
Identification of motile Aeromonas strains with the MicroScan WalkAway system in conjunction with the combo negative type 1S panels.使用MicroScan WalkAway系统结合组合阴性1S型板鉴定运动性气单胞菌菌株。
Appl Environ Microbiol. 2000 Apr;66(4):1764-6. doi: 10.1128/AEM.66.4.1764-1766.2000.
10
Evolving concepts regarding the genus Aeromonas: an expanding Panorama of species, disease presentations, and unanswered questions.气单胞菌属不断演变的概念：物种、疾病表现及未解决问题的全景扩展
Clin Infect Dis. 1998 Aug;27(2):332-44. doi: 10.1086/514652.
Zotero 插件