Huang Zhan, Zeng Zong-Yuan, Peng Han-Wei, Xia Liang-Ping, Hou Jing-Hui
Department of Surgical Oncology, Yuebei People's Hospital, Shaoguan, Guangdong, 512026, PR China.
Ai Zheng. 2003 Sep;22(9):978-81.
BACKGROUND & OBJECTIVE: The prognosis of supraglottic squamous cell carcinoma (SGSCC) was poor. To authors' knowledge, the relationship between inducible nitric oxide synthase (iNOS) and basic fibroblast growth factor (bFGF) and the prognosis of SGSCC has not been addressed to date. This study was designed to evaluate the prognostic significance of these two parameters in SGSCC.
The expression of iNOS and bFGF protein in 64 patients with SGSCC using LSAB method and pathologic imagine analysis system and their association with neck lymph node metastases, recurrence, and prognosis.
(1) The expression of iNOS and bFGF was significantly higher in cervical lymph node positive group (33.2+/-8.8 and 36.1+/-7.8) than in negative group (27.3+/-7.4 and 24.5+/-7.5) with statistical significance. The expression of iNOS and bFGF was higher in recurrence group (36.9+/-1.7 and 34.6+/-2.0) than in no-recurrence group (29.1+/-1.3 and 27.2+/-1.4) with statistical significance. (2) Patients with positive neck lymph node had poorer 5-year survival than those with negative neck lymph node (31.0% vs. 57.1%). Patients with positive iNOS expression had poorer 5-year survival than those with the negative iNOS expression (40.0% vs. 77.8%). However, there was no statistical significance between positive bFGF expression group and negative bFGF expression group. (3) Cox model multivariate regression analysis showed that lymph node status, the expression of iNOS and postoperative radiotherapy were independent prognosis factors, whereas the T staging and the expression of bFGF were not.
The expression of iNOS was one of the independent prognostic factors, but it is necessary to study further for iNOS and bFGF to predict the prognosis of patients with SGSCC using immunohistochemical technique.
声门上型鳞状细胞癌（SGSCC）预后较差。据作者所知，诱导型一氧化氮合酶（iNOS）和碱性成纤维细胞生长因子（bFGF）与SGSCC预后的关系迄今尚未见报道。本研究旨在评估这两个参数在SGSCC中的预后意义。
采用LSAB法及病理图像分析系统检测64例SGSCC患者iNOS和bFGF蛋白的表达，并分析其与颈部淋巴结转移、复发及预后的关系。
（1）iNOS和bFGF在颈部淋巴结阳性组（33.2±8.8和36.1±7.8）的表达明显高于阴性组（27.3±7.4和24.5±7.5），差异有统计学意义。iNOS和bFGF在复发组（36.9±1.7和34.6±2.0）的表达高于未复发组（29.1±1.3和27.2±1.4），差异有统计学意义。（2）颈部淋巴结阳性患者的5年生存率低于阴性患者（31.0%对57.1%）。iNOS表达阳性患者的5年生存率低于阴性患者（40.0%对77.8%）。然而，bFGF表达阳性组与阴性组之间无统计学差异。（3）Cox模型多因素回归分析显示，淋巴结状态、iNOS表达及术后放疗是独立的预后因素，而T分期和bFGF表达不是。
iNOS表达是独立的预后因素之一，但采用免疫组化技术进一步研究iNOS和bFGF以预测SGSCC患者的预后很有必要。
