Gago L April, Saed Ghassan M, Chauhan Subodhsingh, Elhammady Eslam F, Diamond Michael P
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, USA.
Fertil Steril. 2003 Sep;80(3):612-6. doi: 10.1016/s0015-0282(03)00767-2.
To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to modified hyaluronic acid and carboxymethylcellulose (Seprafilm).
University research laboratory.
Human mesothelial and fibroblast cell culture.
MAIN OUTCOME MEASURE(S): Multiplex reverse transcriptase polymerase chain reaction was used to examine control and Seprafilm-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture for levels of messenger RNA from molecules known to be associated with adhesion development (transforming growth factor-beta 1, type I collagen, matrix metalloproteinase-1, matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-1, and tissue plasminogen activator).
RESULT(S): Seprafilm treatment of normal peritoneal fibroblasts, adhesion fibroblasts and mesothelial cells did not alter the expression of markers examined.
CONCLUSION(S): In the absence of a biological effect of Seprafilm on markers known to be involved in postoperative adhesion development, the ability of Seprafilm to reduce postoperative adhesions is most likely caused by its effect as a physical barrier.
描述正常成纤维细胞、粘连成纤维细胞和间皮细胞因暴露于修饰透明质酸和羧甲基纤维素（Seprafilm）而发生的分子变化。
大学研究实验室。
人源间皮细胞和成纤维细胞培养。
采用多重逆转录聚合酶链反应检测对照及经Seprafilm处理的培养中的正常腹膜成纤维细胞、粘连成纤维细胞和间皮细胞中已知与粘连形成相关分子（转化生长因子-β1、I型胶原、基质金属蛋白酶-1、基质金属蛋白酶-2、金属蛋白酶组织抑制剂-1和组织纤溶酶原激活剂）的信使核糖核酸水平。
Seprafilm处理正常腹膜成纤维细胞、粘连成纤维细胞和间皮细胞未改变所检测标志物的表达。
鉴于Seprafilm对已知参与术后粘连形成的标志物无生物学效应，Seprafilm降低术后粘连的能力很可能是其作为物理屏障的作用所致。
