Meyerholz David K, Gallup Jack M, Grubor Branka M, Evans Richard B, Tack Brian F, McCray Paul B, Ackermann Mark R
Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA.
Dev Comp Immunol. 2004 Feb;28(2):171-8. doi: 10.1016/s0145-305x(03)00105-8.
The aim of this study was to define the ontogeny of sheep beta-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.
本研究的目的是分别通过实时定量聚合酶链反应和免疫组织化学法，确定绵羊β-防御素-2（SBD-2）mRNA和肽段在胎儿、新生和成年绵羊选定组织中的个体发生情况。胎儿和新生羔羊的SBD-2组织分布显著高于成年绵羊。在所有年龄段中，肠道中SBD-2 mRNA表达持续存在，而肠道外表达则是散在且微弱的。在成年绵羊中，SBD-2 mRNA水平从空肠向尾侧至直肠逐渐降低，所有年龄组的混合样本也显示出类似趋势。SBD-2免疫反应性细胞主要位于小肠隐窝和绒毛底部以及大肠中数量不多的腺体中。有趣的是，回肠滤泡相关上皮未检测到SBD-2免疫反应性。SBD-2 mRNA和肽段表达在肠道中最高，且组织分布随成熟度逐渐降低。
