Thompson Richard B, Emani Sitaram M, Davis Bryce H, van den Bos Ewout J, Morimoto Yoshihisa, Craig Damian, Glower Donald, Taylor Doris A
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Circulation. 2003 Sep 9;108 Suppl 1:II264-71. doi: 10.1161/01.cir.0000087657.29184.9b.
Multiple cell types are being proposed for cardiac repair, but side-by-side comparisons are lacking. We tested the hypothesis that intracardiac transplantation of autologous bone marrow- or skeletal muscle-derived progenitor cells improve regional heart function to a similar degree.
Thirty-nine New Zealand White rabbits underwent cryoinjury of the left ventricle and simultaneous hind limb bone marrow aspiration or soleus muscle biopsy. Both muscle and bone marrow cells were expanded in vitro. After 2 weeks, 10(8) skeletal muscle (SM group) or bone marrow-derived progenitor cells (BM group) were injected into the cryoinjured region (SM: n=12; BM: n=8). Medium alone was injected into the remaining animals (Control: n=16). Regional systolic function was measured using micromanometry and sonomicrometry at baseline, before, and 4 weeks after cell injection. Cell treatment resulted in a similar degree of improvement in a derivative of stroke work in the SM and BM groups (P=0.0026 and P=0.0085 versus Control, respectively). No significant difference was seen between BM and SM groups (P=0.9). On histology, engrafted cells were found in all of the cell treated animals. Injected myoblasts formed myotubes or muscle cells throughout the scar that expressed slow and fast myosin heavy chain. A subset of bone marrow cells differentiated toward a myogenic phenotype, as indicated by expression of desmin and alpha-sarcomeric actin in the engrafted areas.
Transplantation and myogenic differentiation of bone marrow-derived progenitor cells increased regional systolic heart function after myocardial injury to a similar degree as skeletal myoblasts.
多种细胞类型被提议用于心脏修复，但缺乏并行比较。我们检验了以下假设：自体骨髓或骨骼肌来源的祖细胞心内移植对改善局部心脏功能的程度相似。
39只新西兰白兔接受左心室冷冻损伤并同时进行后肢骨髓抽吸或比目鱼肌活检。肌肉和骨髓细胞均在体外扩增。2周后，将10⁸个骨骼肌细胞（SM组）或骨髓来源的祖细胞（BM组）注入冷冻损伤区域（SM组：n = 12；BM组：n = 8）。仅向其余动物注射培养基（对照组：n = 16）。在基线、细胞注射前及注射后4周，使用微测压法和超声微测法测量局部收缩功能。细胞治疗使SM组和BM组的每搏功衍生物改善程度相似（分别与对照组相比，P = 0.0026和P = 0.0085）。BM组和SM组之间未见显著差异（P = 0.9）。组织学检查发现，所有接受细胞治疗的动物体内均有植入细胞。注入的成肌细胞在整个瘢痕中形成肌管或肌肉细胞，表达慢肌球蛋白重链和快肌球蛋白重链。植入区域中结蛋白和α - 肌动蛋白的表达表明，一部分骨髓细胞向肌源性表型分化。
骨髓来源的祖细胞移植及肌源性分化使心肌损伤后局部收缩性心脏功能的增加程度与骨骼肌成肌细胞相似。
