Zareba T, Hryniewicz W
Zakład Bakteriologii PZH, Warszawie.
Med Dosw Mikrobiol. 1992;44(1-2):1-4.
The study was aimed at evaluation of usefulness of PYR-Wellcome test in bacteriological diagnostic. Results of identification of enterococcal strains by application of the above test were performed on 157 strains of Enterococcus spp., 15 strains of Streptococcus pyogenes and 12 of Streptococcus bovis. Application of test for PYR-ase production results in shortening of diagnostic procedure. Basing on morphological features, including type of hemolysis, lack of catalase production and presence in the bacterial cell of a peptidase hydrolyzing L-pirrolidonyl-beta-naphthylamide, it is possible to classify with high probability the investigated microorganism to the Enterococcus spp. The Shortened procedure is satisfactory for routine identification of enterococcal strains in diagnostic laboratories.
本研究旨在评估PYR-威康测试在细菌学诊断中的实用性。应用上述测试对157株肠球菌属菌株、15株化脓性链球菌和12株牛链球菌进行了鉴定。应用PYR酶产生测试可缩短诊断程序。基于形态学特征,包括溶血类型、过氧化氢酶阴性以及细菌细胞中存在水解L-吡咯烷酮-β-萘酰胺的肽酶,很有可能将所研究的微生物归类为肠球菌属。缩短后的程序对于诊断实验室中肠球菌菌株的常规鉴定是令人满意的。