Szarapińska-Kwaszewska J, Mikucki J
Zakład Mikrobiologii Farmaceutycznej AM, Lodzi.
Med Dosw Mikrobiol. 1992;44(1-2):29-34.
Investigations concerned Escherichia coli nitroreductase in creation of mutagens from non-mutagenic pesticides-derivatives of urea. Three new compounds were studied: N-phenyl-N'-methylurea (IPO 4328), N-methyl,N-(2-hydroxyethyl)-N'phenylurea (IPO 2363), N-(2-hydroxyethyl), N-methyl-N'-(3,4 dichloroethyl) urea, and diurone-3-(3,4 dichlorophenyl)-1,1 dimethylurea. These compounds were incubated in anaerobic conditions with cells of E. coli K-12 (KF) strain and nitrate or nitrite. Using Ames test, mutagenicity of resulting metabolites was investigated. It was found that during incubation of herbicide IPO 4328 with cells of E. coli K-12 (KF) and nitrate, mutagenic product for strain of S. typhimurium TA 1537 is created. Very weak mutagenic metabolite for the same strain was appearing during incubation of herbicide IPO 2363 with cells of E. coli K-12 (KF) in presence of nitrite. Incubation of investigated compounds with E. coli K-12 (KF) cells alone did not result in appearance of mutagenic substances. Thus, role of Escherichia coli in creation of mutagenic compounds from non-mutagenic derivatives of urea consisted of nitrite from nitrate production with participation of nitroreductase, which afterwards in absence of bacteria or action of their enzymes reacted with investigated pesticides.
关于大肠杆菌硝基还原酶在由非诱变农药——尿素衍生物产生诱变剂过程中的研究。研究了三种新化合物：N-苯基-N'-甲基脲（IPO 4328）、N-甲基-N-(2-羟乙基)-N'-苯基脲（IPO 2363）、N-(2-羟乙基)-N-甲基-N'-(3,4-二氯苯基)脲以及敌草隆——3-(3,4-二氯苯基)-1,1-二甲基脲。这些化合物在厌氧条件下与大肠杆菌K-12（KF）菌株的细胞以及硝酸盐或亚硝酸盐一起孵育。使用艾姆斯试验研究了所得代谢产物的诱变性。结果发现，在除草剂IPO 4328与大肠杆菌K-12（KF）菌株的细胞以及硝酸盐孵育期间，产生了针对鼠伤寒沙门氏菌TA 1537菌株的诱变产物。在除草剂IPO 2363与大肠杆菌K-12（KF）菌株的细胞在亚硝酸盐存在下孵育期间，出现了针对同一菌株的非常微弱的诱变代谢产物。仅将所研究的化合物与大肠杆菌K-12（KF）细胞孵育不会导致诱变物质的出现。因此，大肠杆菌在由尿素的非诱变衍生物产生诱变化合物过程中的作用包括在硝基还原酶的参与下由硝酸盐产生亚硝酸盐，随后在没有细菌或其酶的作用的情况下，亚硝酸盐与所研究的农药发生反应。
