de Alborán I Moreno, Baena E, Martinez-A C
Department of Immunology and Oncology (DIO) (Pharmacia-CSIC), Centro Nacional de Biotecnología (CNB-CSIC), Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain.
Cell Death Differ. 2004 Jan;11(1):61-8. doi: 10.1038/sj.cdd.4401319.
C-myc gene is a member of the myc family of proto-oncogenes involved in proliferation, differentiation, and apoptosis. Overexpression of c-myc in fibroblasts causes apoptosis under low serum conditions in a process that requires the interaction of CD95 and CD95L on the surface. We have previously reported an in vivo conditional model to inactivate the c-myc gene in B lymphocytes. Here, we show that c-Myc-deficient primary B lymphocytes are resistant to different apoptotic stimuli. Nonactivated c-Myc-deficient B cells are resistant to spontaneous cell death. Upon activation, c-Myc-deficient B lymphocytes express normal surface levels of activation markers, and show resistance to staurosporine and CD95-induced cell death.
C-myc基因是原癌基因myc家族的成员，参与细胞增殖、分化和凋亡。在成纤维细胞中，c-myc的过表达在低血清条件下会导致细胞凋亡，这一过程需要表面的CD95和CD95L相互作用。我们之前报道了一种在体内使B淋巴细胞中的c-myc基因失活的条件模型。在此，我们表明c-Myc缺陷的原代B淋巴细胞对不同的凋亡刺激具有抗性。未活化的c-Myc缺陷B细胞对自发细胞死亡具有抗性。活化后，c-Myc缺陷的B淋巴细胞表达正常水平的活化标志物表面水平，并对星形孢菌素和CD95诱导的细胞死亡具有抗性。
