转化生长因子β1在结直肠癌组织中的表达与血管生成
TGF beta1 expression and angiogenesis in colorectal cancer tissue.
作者信息
Xiong Bin, Gong Ling-Ling, Zhang Feng, Hu Ming-Bo, Yuan Hong-Yin
机构信息
Department of Oncology, Affiliated Zhongnan Hospital of Wuhan University Wuhan 430071,Hubei,Provice,China.
出版信息
World J Gastroenterol. 2002 Jun;8(3):496-8. doi: 10.3748/wjg.v8.i3.496.
AIM
Transforming growth factor TGF beta1 is involved in a variety of important cellular functions,including cell growth and differentiation, angiogenesis, immune function and extracellular matrix formation. However, the role of TGF beta(1) as an angiogenic factor in colorectal cancer is still unclear. We investigate the relationship between transforming growth factor beta(1) and angiogenesis by analyzing the expression of transforming growth factor TGF beta(1) in colorectal cancer, as well as its association with VEGF and MVD.
METHODS
The expression of TGF beta(1),VEGF, as well as MVD were detected in 98 colorectal cancer by immunohistochemical staining. The relationship between the TGF beta(1) expression and VEGF expression,MVD was evaluated. To evaluate the effect of TGF beta(1) on the angiogenesis of colorectal cancers.
RESULTS
Among 98 cases of colorectal cancer,37 were positive for TGF beta(1) 37.8% 36 for VEGF 36.7% respectively. The microvessel counts ranged from 19 to 139.8, with a mean of 48.7(standard deviation,21.8). The expression of TGF beta(1) was correlated significantly with the depth of invasion, stage of disease, lymph node metastasis, VEGF expression and MVD. Patients in T3-T4, stage III-IV and with lymph node metastasis had much higher expression of TGF beta(1) than patients in T1-T2, stage I-II and without lymph node metastasis (P<0.05). The positive expression rate of VEGF(58.3%) in the TGF-beta(1) positive group is higher than that in the TGF-beta(1) negative group(41.7%, P<0.05). Also, the microvessel count (54+/-18) in TGF-beta(1) positive group is significantly higher than that in TGF-beta(1) negative group(46+/-15, P<0.05). The microvessel count in tumors with both TGF-beta(1) and VEGF positive were the highest (58+/-20 36-140, P<0.05). Whereas that in tumors with both TGF-beta(1) and VEGF negative were the lowest (38+/-16, 19-60, P<0.05).
CONCLUSION
TGF beta(1) might be associated with tumor progression by modulating the angiogenesis in colorectal cancer and TGF beta(1) may be used as a possible biomarker.
目的
转化生长因子β1（TGFβ1）参与多种重要的细胞功能，包括细胞生长与分化、血管生成、免疫功能及细胞外基质形成。然而，TGFβ1作为血管生成因子在结直肠癌中的作用仍不明确。我们通过分析TGFβ1在结直肠癌中的表达及其与血管内皮生长因子（VEGF）和微血管密度（MVD）的关系，来研究转化生长因子β1与血管生成之间的关系。
方法
采用免疫组织化学染色法检测98例结直肠癌组织中TGFβ1、VEGF及MVD的表达情况。评估TGFβ1表达与VEGF表达、MVD之间的关系。以评估TGFβ1对结直肠癌血管生成的影响。
结果
98例结直肠癌中，TGFβ1阳性37例（37.8%），VEGF阳性36例（36.7%）。微血管计数范围为19至139.8，平均为48.7（标准差，21.8）。TGFβ1的表达与浸润深度、疾病分期、淋巴结转移、VEGF表达及MVD显著相关。T3 - T4期、III - IV期及有淋巴结转移的患者TGFβ1表达明显高于T1 - T2期、I - II期及无淋巴结转移的患者（P<0.05）。TGFβ1阳性组VEGF的阳性表达率（58.3%）高于TGFβ1阴性组（41.7%，P<0.05）。此外，TGFβ1阳性组的微血管计数（54±18）明显高于TGFβ1阴性组（46±15，P<0.05）。TGFβ1和VEGF均阳性的肿瘤微血管计数最高（58±20，36 - 140，P<0.05）。而TGFβ1和VEGF均阴性的肿瘤微血管计数最低（38±16，19 - 60，P<0.05）。
结论
TGFβ1可能通过调节结直肠癌的血管生成与肿瘤进展相关，且TGFβ1可能作为一种潜在的生物标志物。
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