Wang Li, Li Jian, Li Qing, Zhang Jian, Duan Xiang-Lin
Life Science College, Hebei Normal University, Shijiazhuang 050016, Hebei Province, China.
World J Gastroenterol. 2003 Sep;9(9):2060-4. doi: 10.3748/wjg.v9.i9.2060.
To study the changes of cell proliferation and apoptosis in rat jejunal epithelium at different ages.
Cell proliferation and apoptosis of the jejunal mucosal and glandulous epithelia from birth to postnatal 12(th) month were observed using immunocytochemistry (ICC), and TUNEL method. The height of villus, the thickness of muscle layer and the number of goblet cells in jejunal mucosal and glandulous epithelia were measured by BeiHang analytic software and analyzed by STAT.
(1) Proliferating cell nuclear antigen (PCNA) positive cells of jejunal glandulous recess were found and increased in number from birth to the postnatal 3(rd) month. The number of PCNA positive cells peaked in the postnatal 3(rd) month, and decreased from then on. (2) The number of apoptotic cells also peaked in the postnatal 3(rd) month, showing a similar trend to that of the PCNA positive cells. (3) The height of jejunal villus increased after birth, peaked in the postnatal 3(rd) month and decreased from then on. The jejunal muscle layer became thicker in the postnatal 3(rd) week and the postnatal 12(th) month. The number of goblet cells of the jejunal mucosal and glandulous epithelia had a linear correlation with age.
(1) PCNA positive cells are distributed in the jejunal glandulous recess. (2) Apoptotic cell number peaks in the postnatal 3(rd) month, indicating that cell proliferation and apoptosis are developed with the formation of digestive metabolism as rat grows to maturity. (3) The thickness of jejunal muscle layer increases to a maximum in the postnatal 3(rd) week, which may be related to the change in diet from milk to solid food. (4) The number of goblet cells increases rapidly in the postnatal 3(rd) week, probably due to ingestion of solid food.
研究不同年龄大鼠空肠上皮细胞增殖和凋亡的变化。
采用免疫细胞化学法（ICC）和TUNEL法观察出生至生后12个月大鼠空肠黏膜和腺上皮细胞的增殖和凋亡情况。应用北航分析软件测量空肠黏膜和腺上皮绒毛高度、肌层厚度及杯状细胞数量，并进行统计学分析。
（1）空肠腺隐窝增殖细胞核抗原（PCNA）阳性细胞出生时即存在，生后3个月数量增多，PCNA阳性细胞数量在生后3个月达到峰值，之后逐渐减少。（2）凋亡细胞数量也在生后3个月达到峰值，与PCNA阳性细胞变化趋势相似。（3）空肠绒毛高度出生后增加，生后3个月达到峰值，之后下降。空肠肌层在生后3周和12个月时增厚。空肠黏膜和腺上皮杯状细胞数量与年龄呈线性相关。
（1）PCNA阳性细胞分布于空肠腺隐窝。（2）凋亡细胞数量在生后3个月达到峰值，提示随着大鼠生长至成熟，细胞增殖和凋亡随消化代谢的形成而发展。（3）空肠肌层厚度在生后3周增至最大，这可能与饮食从乳类向固体食物转变有关。（4）杯状细胞数量在生后3周迅速增加，可能与摄入固体食物有关。
