全反式维甲酸对人肝癌细胞增殖的抑制作用。
Inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell proliferation.
作者信息
Piao Yun-Feng, Shi Yang, Gao Pu-Jun
机构信息
Department of Gastroenterology, the First Hospital of Jilin University, Changchun, 130021, Jilin Province, China.
出版信息
World J Gastroenterol. 2003 Sep;9(9):2117-20. doi: 10.3748/wjg.v9.i9.2117.
AIM
To study the inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell line SMMC-7721 and to explore the mechanism of its effect.
METHODS
SMMC-7721 cells were divided into two groups, one treated with all-trans retinoic acid (ATRA) for 5 days and the other as a control group. Light microscope and electron microscope were used to observe the morphological changes. Telomerase activity was analyzed with silver-stained telomere repeated assay protocal (TRAP). Expression of Caspase-3 was demonstrated with western blot.
RESULTS
ATRA-treated cells showed differentiation features including small and pyknotic nuclei, densely stained chromatin and fewer microvilli. Besides, ATRA could inhibit the activity of telomerase, promote the expression of Caspase-3 and its activation.
CONCLUSION
Telomerase activity and Caspase-3 expression are changed in human hepatocellular carcinoma cell line SMMC-7721 treated with all-trans retinioc acid. The inhibition of telomerase activity and the activation of Caspase-3 may be the key steps through which ATRA inhibits the proliferation of SMMC-7721 cell line.
目的
研究全反式维甲酸对人肝癌细胞系SMMC - 7721的抑制作用，并探讨其作用机制。
方法
将SMMC - 7721细胞分为两组，一组用全反式维甲酸（ATRA）处理5天，另一组作为对照组。用光镜和电镜观察形态学变化。采用银染端粒重复序列分析方法（TRAP）分析端粒酶活性。用蛋白质免疫印迹法检测Caspase - 3的表达。
结果
经ATRA处理的细胞呈现出分化特征，包括细胞核小而固缩、染色质致密、微绒毛减少。此外，ATRA可抑制端粒酶活性，促进Caspase - 3的表达及其激活。
结论
全反式维甲酸处理的人肝癌细胞系SMMC - 7721中端粒酶活性和Caspase - 3表达发生改变。抑制端粒酶活性和激活Caspase - 3可能是ATRA抑制SMMC - 7721细胞系增殖的关键步骤。
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