Lu Yun, Wu Li-Qun
Department of Hepato-biliary Vascular Surgery, Medical College, Qingdao University, 16 Jiangsu Road, Qingdao 266003, Shandong Province, China.
World J Gastroenterol. 2003 Sep;9(9):2121-4. doi: 10.3748/wjg.v9.i9.2121.
To study the therapeutic effectiveness of intra-tumor injection of Xiao-Zhi-Ling(XZL) on transplanted hepatoma in rats.
Sixty rats were divided into 3 groups (groups S, X and E), 20 in each. Different drugs were injected into the implanted hepatoma (Group S with 0.2 ml saline as control, group X with 0.2 ml XZL, group E with 0.2 ml ethanol). After 3 days and 8 days respectively, we detected the hepatoma volume (HV), the level of albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in serum, and the expression of proliferating cell nuclear antigen (PCNA) in hepatoma.
The results were obtained after 3 days, the HVs in groups X and E were smaller than those in group S (group X vs S P=0.010(), group E vs S P=0.002(), P<0.05). The levels of ALT and AST in group S and X were lower than those in group E (ALT Group S vs E P=0.019(), group X vs E P=0.003(), P<0.05; AST group X vs E P=0.002(), P<0.05). The levels of ALP and PCNA labeling index in group X were lower than those in group S and E (ALP group X vs S P=0.000(), group X vs E P=0.000(). P<0.05; PCNA group X vs S P=0.008(), group X vs E P=0.048(), P<0.05). The levels of creatinine in group S were lower than those in group E (group S vs E P=0.017, P<0.05). The degree of tumor necrosis in group S was lower than those in groups X and E (group S vs X P=0.006(), group S vs E P=0.006(), P<0.05). After 8 days, the HVs in groups X and E were smaller than those in group S (group X vs S P=0.007(), group E vs S P=0.004(), P<0.05). The difference of HVs between groups X and E was not significant. The levels of albumin, ALT, AST and creatinine in group X were not higher than those in other groups, the levels of ALP and PCNA in group X were lower than those in groups S and E (ALP group X vs E P=0.006() P<0.05; PCNA group X vs S P=0.044(), group X vs E P=0.021(), P<0.05). The degree of tumor necrosis in group S was lower than that in groups X and E (group S vs X P=0.001(), group S vs E P=0.002(), P<0.05).
The therapeutic effectiveness of intra-tumor injection of XZL and ethanol on implanted hepatoma is obvious, but the toxicity of XZL on liver function is markedly lower than that of group E, at the same time XZL can inhibit the growth of tumor. XZL is relatively better and safer than ethanol in intra-tumor injection therapy.
研究瘤内注射消痔灵（XZL）对大鼠移植性肝癌的治疗效果。
将60只大鼠分为3组（S组、X组和E组），每组20只。将不同药物注入植入的肝癌内（S组注射0.2 ml生理盐水作为对照，X组注射0.2 ml消痔灵，E组注射0.2 ml乙醇）。分别在3天和8天后，检测肝癌体积（HV）、血清中白蛋白、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）水平，以及肝癌中增殖细胞核抗原（PCNA）的表达。
3天后结果显示，X组和E组的肝癌体积小于S组（X组与S组比较P = 0.010（），E组与S组比较P = 0.002（），P < 0.05）。S组和X组的ALT和AST水平低于E组（ALT：S组与E组比较P = 0.019（），X组与E组比较P = 0.003（），P < 0.05；AST：X组与E组比较P = 0.002（），P < 0.05）。X组的ALP和PCNA标记指数水平低于S组和E组（ALP：X组与S组比较P = 0.000（），X组与E组比较P = 0.000（），P < 0.05；PCNA：X组与S组比较P = 0.008（），X组与E组比较P = 0.048（），P < 0.05）。S组的肌酐水平低于E组（S组与E组比较P = 0.017，P < 0.05）。S组的肿瘤坏死程度低于X组和E组（S组与X组比较P = 0.006（），S组与E组比较P = 0.006（），P < 0.05）。8天后，X组和E组的肝癌体积小于S组（X组与S组比较P = 0.007（），E组与S组比较P = 0.004（），P < 0.05）。X组和E组之间的肝癌体积差异不显著。X组的白蛋白、ALT、AST和肌酐水平不高于其他组，X组的ALP和PCNA水平低于S组和E组（ALP：X组与E组比较P = 0.006（），P < 0.05；PCNA：X组与S组比较P = 0.044（），X组与E组比较P = 0.021（），P < 0.05）。S组的肿瘤坏死程度低于X组和E组（S组与X组比较P = 0.001（），S组与E组比较P = 0.002（），P < 0.05）。
瘤内注射消痔灵和乙醇对植入性肝癌的治疗效果明显，但消痔灵对肝功能的毒性明显低于E组，同时消痔灵可抑制肿瘤生长。在瘤内注射治疗中，消痔灵比乙醇相对更好且更安全。
