Wen Zhi-bin, Xiong Shi-long, He Xiao-fan, He Mei-xia, He Shi-lin, Xie Qin-zhi, Jian Zai-fu, Chen Fang-ping, Xiao Bo, Yang Qi-dong
Thrombosis and Hemostasis Lab, Department of Physiology, Xiangya Medical University, Central-South University, Changsha 410078, Hunan, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2003 Sep;15(9):529-31.
To establish the possible relationship between some coagulation factors and the onset of acute cerebral infarction (ACI).
The study population consisted of 71 patients with ACI confirmed by CT and 50 age-matched healthy volunteers. Blood samples were obtained during the onset period of ACI. Tissue factor (TF) and tissue factor pathway inhibitor (TFPI) activity in plasma were assayed with the chromogenic assay. Plasma TF and TFPI antigen were measured with enzyme linked immunoadsorbent assay (ELISA). Plasma F VII coagulation activity (F VII: C) and F VIII coagulation activity (F VIII: C) were developed in the one-stage system. Plasma prothrombin (FII) was determined with Ecarin assay. Plasma fibrinogen (Fbg) was measured with thrombin assay. Plasma antithrombin III activity (ATIII) was determined using heparin cofactor activity assay.
Compared with the control, plasma TF activity and antigen in patients with ACI were significantly higher (both P<0.05). But plasma TFPI activity and antigen were remarkably lower in the ACI group (both P<0.05). Plasma F VII: C was significantly higher (P<0.01), and F VIII: C was markedly lower (P<0.05). Plasma FII was remarkably higher (P<0.01). Similarly the Fbg was significantly higher in the ACI than that in the control group (P<0.01), whereas ATIII was significantly lower (P<0.01).
The initiation of TF pathway is contributed to the onset of ACI and the blood is in hypercoagulable state during the early period of ACI.
探讨某些凝血因子与急性脑梗死（ACI）发病之间的可能关系。
研究对象包括71例经CT证实的ACI患者和50例年龄匹配的健康志愿者。在ACI发病期采集血样。采用发色底物法检测血浆组织因子（TF）和组织因子途径抑制物（TFPI）活性。采用酶联免疫吸附测定（ELISA）法检测血浆TF和TFPI抗原。在一步法体系中检测血浆F VII凝血活性（F VII：C）和F VIII凝血活性（F VIII：C）。采用蛇毒凝血酶法测定血浆凝血酶原（FII）。采用凝血酶法测定血浆纤维蛋白原（Fbg）。采用肝素辅因子活性测定法测定血浆抗凝血酶III活性（ATIII）。
与对照组相比，ACI患者血浆TF活性和抗原显著升高（均P<0.05）。但ACI组血浆TFPI活性和抗原显著降低（均P<0.05）。血浆F VII：C显著升高（P<0.01），F VIII：C显著降低（P<0.05）。血浆FII显著升高（P<0.01）。同样，ACI组Fbg显著高于对照组（P<0.01），而ATIII显著降低（P<0.01）。
TF途径的启动促成了ACI的发病，且在ACI早期血液处于高凝状态。
