Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea.
作者信息
Chai Jong-Yil, Lin Aifen, Shin Eun-Hee, Oh Myoung-Don, Han Eun-Taek, Nan Ho-Woo, Lee Soon-Hyung
机构信息
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
出版信息
Korean J Parasitol. 2003 Sep;41(3):147-54. doi: 10.3347/kjp.2003.41.3.147.
Abstract
Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.
摘要
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本文引用的文献
1
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J Parasitol. 2000 Aug;86(4):716-23. doi: 10.1645/0022-3395(2000)086[0716:ISIPAN]2.0.CO;2.
2
Characterisation of Toxoplasma gondii isolates using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) of the non-coding Toxoplasma gondii (TGR)-gene sequences.利用聚合酶链反应（PCR）和非编码型刚地弓形虫（TGR）基因序列的限制性片段长度多态性（RFLP）对刚地弓形虫分离株进行鉴定。
Int J Parasitol. 2000 Jun;30(7):853-8. doi: 10.1016/s0020-7519(00)00059-x.
3
In vitro isolation and characterization of bovine Neospora caninum in Korea.
Vet Parasitol. 2000 Jun 10;90(1-2):147-54. doi: 10.1016/s0304-4017(00)00224-7.
4
Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis.通过GRA6基因序列分析对弓形虫菌株进行分子分型
Int J Parasitol. 2000 Apr 24;30(5):637-42. doi: 10.1016/s0020-7519(00)00036-9.
5
A toxoplasmic uveitis case of a 60-year-old male in Korea.韩国一名60岁男性的弓形虫性葡萄膜炎病例。
Korean J Parasitol. 2000 Mar;38(1):29-31. doi: 10.3347/kjp.2000.38.1.29.
6
Pathogenesis of toxoplasmic retinochoroiditis.弓形虫性视网膜脉络膜炎的发病机制。
Parasitol Today. 1999 Feb;15(2):51-7. doi: 10.1016/s0169-4758(98)01377-5.
7
Toxoplasma gondii antibody titers in sera of children admitted to the Seoul National University Children's Hospital.首尔国立大学儿童医院收治儿童血清中的弓形虫抗体滴度。
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8
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Int J Parasitol. 1998 Jul;28(7):1041-51. doi: 10.1016/s0020-7519(98)00074-5.
9
Experimental infection of murine splenic lymphocytes and granulocytes with Toxoplasma gondii RH tachyzoites.用刚地弓形虫RH速殖子对小鼠脾淋巴细胞和粒细胞进行实验性感染。
Korean J Parasitol. 1997 Jun;35(2):79-85. doi: 10.3347/kjp.1997.35.2.79.
10
Foodborne outbreaks of human toxoplasmosis.食源性人类弓形虫病暴发。
J Infect Dis. 1997 May;175(5):1280-2. doi: 10.1086/593702.
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