Chai Jong-Yil, Lin Aifen, Shin Eun-Hee, Oh Myoung-Don, Han Eun-Taek, Nan Ho-Woo, Lee Soon-Hyung
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
Korean J Parasitol. 2003 Sep;41(3):147-54. doi: 10.3347/kjp.2003.41.3.147.
Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.
从一名眼部疾病患者的血液中分离出了刚地弓形虫速殖子，并通过在小鼠体内进行腹腔接种，在实验室中成功传代一年多。分离出的寄生虫被命名为韩国分离株-1（KI-1），并将其特征与RH株进行了比较，RH株是一种源自一名患脑炎儿童的著名强毒株。KI-1的形态、致病性、感染性和细胞培养特征与RH株相似。抗刚地弓形虫单克隆抗体（mAb）Tg563可检测到RH和KI-1的抗原，该抗体针对主要表面蛋白SAG1（30 kDa），而抗犬新孢子虫单克隆抗体12B4未观察到反应。KI-1被确认为刚地弓形虫的一个分离株。韩国首次报道了对当地刚地弓形虫分离株的长期实验室保存和特征描述。
