Liao S S, Guan K, Cui F Z, Shi S S, Sun T S
Biomaterials Laboratory, Department of Materials Science & Engineering, Tsinghua University, Beijing, China.
Spine (Phila Pa 1976). 2003 Sep 1;28(17):1954-60. doi: 10.1097/01.BRS.0000083240.13332.F6.
A new mineralized collagen matrix combined with or without growth factor was used for the posterolateral spinal fusion in the rabbit lumbar spine.
The availability of a new osteoconductive matrix with or without recombinant osteoinductive growth factors offers a possible alternative to the use of autogenous bone for grafting indications. This study evaluated the bone-forming activity of the biomimetic matrix: nano-hydroxyapatite/collagen/polylactic acid (nHAC/PLA) combined without or with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a rabbit posterolateral spinal fusion.
Many bone grafting materials such as titanium alloy, ceramics, and polymers were used to repair bony defects. However, each has specific disadvantaged. The permanent implantation still has possibility to be eroded in vivo, which is caused by late breakdown and abscess formation. The acidic outcome of polymer biodegradation was also negatively affected in the later-stage results of bone repair. It needed a promising material for an alternative to the use of autogenous bone for grafting indications.
Sixty-four rabbits were randomly divided into four groups: autologous iliac crest bone group (ACB), nHAC/PLA composite group (nHAC/PLA), autologous iliac crest bone mixed with nHAC/PLA composite group (ACB + nHAC/PLA), nHAC/PLA composite with recombinant human BMP-2 group (nHAC/PLA + rhBMP-2). The lumbar intertransverse process fusions were assessed by manual palpation, radiographic, histologic, and mechanical strength, and scanning electronic microscopy (SEM) in a 10-week observation.
Optimal formulations of the ACB + nHAC/PLA and nHAC/PLA + rhBMP-2 groups were shown to perform similar to ACB in both the fusion ratio and mechanical strength in the 6 and 10 weeks after surgery. From the microstructure analysis of the samples, there was no negative effect when the compound implanted this composite with autogenous iliac crest, and there was also new bone-like tissue formation implanted this composite without combined rhBMP-2 early at the second week after surgery.
This study shows the effective results of nHAC/PLA in rabbit posterolateral spinal fusion combined with rhBMP-2. It is an alternative method to autograft by compounding this osteoconductive matrix with growth factors.
一种新型矿化胶原基质，添加或不添加生长因子，用于兔腰椎后外侧脊柱融合。
一种新型的具有或不具有重组骨诱导生长因子的骨传导基质，为自体骨移植提供了一种可能的替代方法。本研究评估了仿生基质：纳米羟基磷灰石/胶原/聚乳酸（nHAC/PLA）在添加或不添加重组人骨形态发生蛋白-2（rhBMP-2）的情况下，在兔后外侧脊柱融合中的成骨活性。
许多骨移植材料，如钛合金、陶瓷和聚合物，被用于修复骨缺损。然而，每种材料都有特定的缺点。永久性植入物在体内仍有可能被侵蚀，这是由后期分解和脓肿形成引起的。聚合物生物降解的酸性产物在骨修复的后期结果中也有负面影响。需要一种有前景的材料来替代自体骨移植。
64只兔子随机分为四组：自体髂嵴骨组（ACB）、nHAC/PLA复合材料组（nHAC/PLA）、自体髂嵴骨与nHAC/PLA复合材料混合组（ACB + nHAC/PLA）、nHAC/PLA复合材料与重组人BMP-2组（nHAC/PLA + rhBMP-2）。通过手动触诊、影像学、组织学、力学强度以及扫描电子显微镜（SEM）对腰椎横突间融合进行了为期10周的观察评估。
ACB + nHAC/PLA组和nHAC/PLA + rhBMP-2组的最佳配方在术后6周和10周的融合率和力学强度方面表现与ACB组相似。从样本的微观结构分析来看，当该复合材料与自体髂嵴联合植入时没有负面影响，并且在术后第二周早期，该复合材料在未联合rhBMP-2的情况下植入也有新的类骨组织形成。
本研究表明nHAC/PLA在兔后外侧脊柱融合中联合rhBMP-2具有有效的结果。通过将这种骨传导基质与生长因子复合，是自体移植的一种替代方法。
