Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Centre, Academy of Military Medical Sciences, 27 Taiping Road, Beijing 100850, People's Republic of China.
J Tissue Eng Regen Med. 2012 Apr;6(4):325-36. doi: 10.1002/term.445. Epub 2011 Jul 13.
Spinal fusion is routinely performed to treat low back pain caused by degeneration of intervertebral discs. An autologous bone graft derived from the iliac crest is the standard procedure used for spinal fusion. However, several shortcomings, including pseudarthrosis, pain and the need for blood transfusion are known to be associated with the procedure. Our study analysed the effectiveness of a new mineralized collagen matrix, nano-hydroxyapatite-collagen-polylactic acid (nHAC-PLA), combined with autologous adipose-derived mesenchymal stem cells (ADMSCs) as a graft material for posterolateral spinal fusion in a rabbit model. Forty rabbits were randomly divided into four groups: autologous iliac crest bone group (ACB), nHAC-PLA composite group (nHAC-PLA), autologous iliac crest bone mixed with nHAC-PLA composite group (ACB + nHAC-PLA), and nHAC-PLA composite combined with ADMSCs (ADMSCs + nHAC-PLA). The viability and the proliferation of the ADMSCs seeded on the scaffolds were evaluated by live/dead kit and MTT assay in vitro, respectively. Lumbar posterolateral fusions were assessed by manual palpation, radiographical and histological procedures, mechanical strength and scanning electronic microscopy (SEM) in 10 weeks of observation. The results showed that the rate of fusion was significantly higher in the ACB and ADMSCs + nHAC-PLA groups than that in the nHAC-PLA and ACB + nHAC-PLA groups. It was not significantly higher in the ACB group than in the ADMSCs + nHAC-PLA group. From microstructural analysis of the samples using histological staining methods, there was more new bone-like tissue formation in the ACB and ADMSCs + nHAC-PLA groups than that in the other two groups at the 10th postoperative week. Our study demonstrated the effective impact of nHAC-PLA combined with ADMSCs in rabbit posterolateral spinal fusion.
脊柱融合术通常用于治疗由椎间盘退变引起的腰痛。自体髂嵴骨移植物是用于脊柱融合的标准程序。然而，该程序已知存在一些缺点，包括假关节形成、疼痛和需要输血。我们的研究分析了一种新的矿化胶原基质，纳米羟基磷灰石-胶原-聚乳酸（nHAC-PLA）与自体脂肪来源间充质干细胞（ADMSCs）结合作为兔模型后路脊柱融合的移植物材料的有效性。40 只兔子被随机分为四组：自体髂嵴骨组（ACB）、nHAC-PLA 复合材料组（nHAC-PLA）、自体髂嵴骨混合 nHAC-PLA 复合材料组（ACB+nHAC-PLA）和 nHAC-PLA 复合材料联合 ADMSCs（ADMSCs+nHAC-PLA）。通过活/死试剂盒和 MTT 检测分别评估 ADMSCs 接种在支架上的活力和增殖。在 10 周的观察期内，通过手动触诊、影像学和组织学程序、机械强度和扫描电子显微镜（SEM）评估腰椎后路融合情况。结果表明，ACB 和 ADMSCs+nHAC-PLA 组的融合率明显高于 nHAC-PLA 和 ACB+nHAC-PLA 组。ACB 组与 ADMSCs+nHAC-PLA 组相比，融合率并没有明显升高。通过组织学染色方法对样本进行微观结构分析，在第 10 周术后，ACB 和 ADMSCs+nHAC-PLA 组比其他两组有更多的新骨样组织形成。我们的研究表明，nHAC-PLA 联合 ADMSCs 对兔后路脊柱融合有显著的促进作用。
