Paulin-Levasseur M
Department of Biology, University of Ottawa, Ont., Canada.
Biochem Cell Biol. 1992 Oct-Nov;70(10-11):842-8. doi: 10.1139/o92-132.
Major constituents of the cytoskeleton and the nuclear matrix, cytoplasmic intermediate filament subunits and nuclear lamins belong to a multigene family of proteins whose function is poorly understood. It has now become a general contention that important clues to the physiological roles of these proteins may reside in their developmental and tissue-specific expression patterns, as well as their cellular organization. The present review brings into focus experimental strategies that have been developed, over the past few years, to gain insights into the cellular mechanisms regulating the molecular polymorphism and supramolecular assembly of intermediate filaments. In this context new concepts are discussed that may be pivotal for the orientation of future studies on intermediate filament proteins.
细胞骨架和核基质的主要成分、细胞质中间丝亚基和核纤层蛋白属于一个多基因家族的蛋白质，其功能尚不清楚。现在人们普遍认为，这些蛋白质生理作用的重要线索可能存在于它们的发育和组织特异性表达模式以及细胞组织中。本综述重点介绍了过去几年中为深入了解调节中间丝分子多态性和超分子组装的细胞机制而开发的实验策略。在这方面，讨论了一些新概念，这些概念可能对未来中间丝蛋白研究的方向至关重要。
