使用非放射性方法检测恶性疟原虫中的端粒酶活性。
Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method.
作者信息
Rubiano Claudia C, Wasserman Moises
机构信息
Laboratorio Bioquímica, Instituto Nacional de Salud, Bogotá, Colombia.
出版信息
Mem Inst Oswaldo Cruz. 2003 Jul;98(5):693-5. doi: 10.1590/s0074-02762003000500018. Epub 2003 Sep 8.
Abstract
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
摘要
采用一种简单、快速且灵敏的方法来检测恶性疟原虫中的端粒酶活性。通过电泳和用SYBR-绿I染色对端粒重复序列扩增协议（TRAP检测法）进行了改进，以检测10至10⁷个疟原虫范围内的端粒酶活性。这可能是确定不同类型非肿瘤细胞中端粒酶活性的一种有用方法。
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