Szatmari I, Aradi J
Department of Biochemistry and Molecular Biology, Medical and Health Science Center, University of Debrecen, H-4012 Debrecen, Hungary.
Nucleic Acids Res. 2001 Jan 15;29(2):E3. doi: 10.1093/nar/29.2.e3.
The Telomeric Repeat Amplification Protocol (TRAP) and its modified versions (including ours, TP-TRAP) change the size and/or the ratio of the telomerase products in the amplification stage of the assay. Based on our recently published method we developed a new TRAP. This method ensures that the number of telomeric repeats present in the original telomerase products does not change on PCR amplification. The usefulness of the method was proved with amplification of chemically synthesized telomerase products and a newly designed telomerase substrate oligonucleotide. This is the first report in which the PCR products directly reflect the size distribution of telomerase products generated by the enzyme.
端粒重复序列扩增法(TRAP)及其改进版本(包括我们的TP - TRAP)在检测的扩增阶段会改变端粒酶产物的大小和/或比例。基于我们最近发表的方法,我们开发了一种新的TRAP。该方法可确保原始端粒酶产物中存在的端粒重复序列数量在PCR扩增过程中不会改变。通过化学合成的端粒酶产物和新设计的端粒酶底物寡核苷酸的扩增,证明了该方法的有效性。这是第一份PCR产物直接反映该酶产生的端粒酶产物大小分布的报告。