Xu Xiaoyan, Xiang Wenpei, Chen Hanping
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
J Huazhong Univ Sci Technolog Med Sci. 2003;23(2):184-6. doi: 10.1007/BF02859952.
To study the expression of placental isoferritin (PLF) in placental tissue of pregnancy-induced hypertension (PIH) and the relationship between the level of expression of PLF and the amount of vascular cell adhesion molecule-1 (VCAM-1) in serum, immunohistochemical technique was used to detect the expression of PLF in placenta tissue in 45 PIH patients (PIH group) and 15 normal pregnant women (normal group). High resolution pathological image analysis system (HPIAS-100) was employed to determine the quantity of PLF. The VCAM-1 in serum was examined by enzyme linked immunoabsorbent assay (ELISA). The results showed that the levels of PLF expressions in moderate and severe PIH patients were significantly lower than that of normal group (P < 0.01). The serum VCAM-1 was significantly decreased in PIH group (1310 +/- 177 rho/ng/ml) than that of normal group (609 +/- 72 rho/ng/ml, P < 0.01). The significant negative correlation existed between the expression of PLF in placental tissue and the serum VACM-1 (r = -0.58, P < 0.01). It was concluded that the level of PLF expression in PIH decreases and is negatively correlated with the amount of serum VCAM-1, indicating that these may be involved in the pathogenesis of PIH.
为研究胎盘铁蛋白（PLF）在妊娠高血压综合征（PIH）胎盘组织中的表达及PLF表达水平与血清中血管细胞黏附分子-1（VCAM-1）含量的关系，采用免疫组织化学技术检测45例PIH患者（PIH组）和15例正常孕妇（正常组）胎盘组织中PLF的表达。运用高分辨率病理图像分析系统（HPIAS-100）测定PLF的含量。采用酶联免疫吸附测定法（ELISA）检测血清中的VCAM-1。结果显示，中度和重度PIH患者的PLF表达水平显著低于正常组（P<0.01）。PIH组血清VCAM-1（1310±177ρ/ng/ml）显著低于正常组（609±72ρ/ng/ml，P<0.01）。胎盘组织中PLF的表达与血清VACM-1之间存在显著负相关（r=-0.58，P<0.01）。结论：PIH中PLF表达水平降低，且与血清VCAM-1含量呈负相关，提示其可能参与PIH的发病机制。
