Sedlak J, Reinerova M, Hunakova L, Ausch C, Chorvath B, Rosen H, Moroz C
Cancer Research Institute, Bratislava, Slovakia.
Cancer Lett. 1995 Jul 20;94(1):101-6. doi: 10.1016/0304-3835(95)03831-g.
the ability of the breast cancer-associated placental acid isoform of ferritin (p43-PLF) to modulate cell surface expression of HLA, CD59 (protectin) and CD66 antigen (adhesion antigen related to CEA) was examined.
the expression of these antigens in human breast carcinoma cell lines BT-20, T47D and MDA-MB-468 was determined with the aid of flow cytometry and monoclonal antibodies.
PLF induced a transient up-regulation followed by a down regulation of cell surface protectin (CD59 antigen) on the cell surface of T47D and to a lesser extent, BT-20 human breast carcinoma cell lines. Furthermore, PLF down-regulated cell surface expression of CEA-related CD66 antigen on both these cell lines. No PLF-induced alterations of protectin, CD66 antigen and HLA class I antigen were found on the MDA-MB-468 breast cancer cell line.
breast cancer-associated p43 induces alterations of the expression of cell surface molecules in breast cancer cells which could have an effect on the modulation of cancer cell adhesive interactions.
研究乳腺癌相关的胎盘型铁蛋白酸性异构体（p43-PLF）调节人类白细胞抗原（HLA）、CD59（保护素）和CD66抗原（与癌胚抗原相关的黏附抗原）细胞表面表达的能力。
借助流式细胞术和单克隆抗体，测定这些抗原在人乳腺癌细胞系BT-20、T47D和MDA-MB-468中的表达。
PLF可诱导T47D细胞表面的保护素（CD59抗原）出现短暂上调，随后下调，在BT-20人乳腺癌细胞系中程度稍轻。此外，PLF可下调这两种细胞系中与癌胚抗原相关的CD66抗原的细胞表面表达。在MDA-MB-468乳腺癌细胞系中未发现PLF诱导的保护素、CD66抗原和HLA I类抗原的改变。
乳腺癌相关的p43可诱导乳腺癌细胞表面分子表达的改变，这可能对癌细胞黏附相互作用的调节产生影响。
