Năcescu N, Israil A, Cedru C, Caplan D
Cantacuzino Institute, Vibrio Laboratory, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1992 Jul-Sep;51(3):147-56.
Considering the possible correlation between hemolytic and enterotoxigenic properties of Aeromonas strains mentioned in the literature, in the present work we studied the practical value of the hemolysis tests in the diagnosis of Aeromonas strains by using comparatively the hemolysis tube tests (with goat and sheep erythrocytes suspensions) as well as the technique on blood agar in aerobic conditions. There were studied comparatively 230 Aeromonas strains (different species: A. hydrophila, A. sobriae, A. caviae, A. veronii, A. salmonicida) isolated from different sources (meat products, fish of fresh waters, mussels, sea water, pipe water, diarrhoeal disease and animal faeces). The comparison among the used tests for proving the hemolytic activity of Aeromonas strains showed that: 61.89% and 56.51% from the total number of Aeromonas strains were hemolytic in the tube tests with 1% goat and 1% sheep erythrocytes suspensions respectively after 48 hrs followed by 51.72% and 48.25% strains by the 5% goat and 5% sheep blood agar plates respectively in anaerobiosis and after 48 hrs incubation at 37 degrees C. The highest incidence of hemolysin presence was pointed out in motile Aeromonas strains (A. hydrophila--60.11% and A. sobria--56.52%). Our results showing a high frequency of hemolytic activity among Aeromonas strains isolated especially in meat products are suggesting a possible correlation between the pathogenic potential and the hemolytic activity and are pleading for introduction of this test in the diagnosis of Aeromonas species.
考虑到文献中提到的气单胞菌菌株溶血特性和产肠毒素特性之间可能存在的相关性，在本研究中，我们通过比较溶血试管试验（使用山羊和绵羊红细胞悬液）以及在需氧条件下血琼脂平板上的技术，研究了溶血试验在气单胞菌菌株诊断中的实际价值。对从不同来源（肉制品、淡水鱼、贻贝、海水、自来水、腹泻病患者和动物粪便）分离出的230株气单胞菌菌株（不同种：嗜水气单胞菌、温和气单胞菌、豚鼠气单胞菌、维罗纳气单胞菌、杀鲑气单胞菌）进行了比较研究。对用于证明气单胞菌菌株溶血活性的试验进行比较后发现：在使用1%山羊和1%绵羊红细胞悬液的试管试验中，48小时后，分别有61.89%和56.51%的气单胞菌菌株具有溶血活性；在厌氧条件下，于37℃孵育48小时后，在5%山羊和5%绵羊血琼脂平板上分别有51.72%和48.25%的菌株具有溶血活性。在运动性气单胞菌菌株（嗜水气单胞菌——60.11%，温和气单胞菌——56.52%）中溶血素存在的发生率最高。我们的结果表明，特别是在肉制品中分离出的气单胞菌菌株中溶血活性频率较高，这表明致病潜力与溶血活性之间可能存在相关性，并主张将该试验引入气单胞菌属的诊断中。
