Nakano H, Kameyama T, Venkateswaran K, Kawakami H, Hashimoto H
Department of Food Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University.
Microbiol Immunol. 1990;34(5):447-58. doi: 10.1111/j.1348-0421.1990.tb01027.x.
A year-long survey on the distribution of motile Aeromonas species in the surface waters of riverine and marine environments was conducted. The filtered membranes were directly placed onto the modified Pril-xylose-ampicillin agar for the enumeration of Aeromonas species. High counts of motile aromonads were found in riverine stations and this bacterial population was also observed in significant quantities in polluted marine samples. In the identification of 2,444 isolates, three species of motile Aeromonas were observed. A. caviae (43%) was prevalent followed by A. sobria (35%) and A. hydrophila (20%). A. hydrophila was high in clean riverine samples, A. sobria was predominantly isolated from a stagnant water sampling area, and A. caviae was distributed more in marine samples. Statistical analyses suggested that the densities of Aeromonas were related to the cumulative effect of various physicochemical parameters rather than to a single factor. Among the species of Aeromonas, A. hydrophila, and A. sobria were highly hemolytic whereas only 11% of A. caviae were observed to lyse sheep erythrocytes. Suckling-mouse assay was performed to elucidate the enterotoxicity of motile aeromonads and 21% of the tested strains (one A. caviae strain) were found to produce enterotoxin.
开展了一项为期一年的关于河流和海洋环境表层水中运动性气单胞菌属物种分布的调查。将过滤后的膜直接置于改良的普里木糖 - 氨苄青霉素琼脂上，用于气单胞菌属物种的计数。在河流监测站发现了大量的运动性气单胞菌，并且在受污染的海洋样本中也观察到了大量的该细菌种群。在对2444株分离菌的鉴定中，观察到了三种运动性气单胞菌。豚鼠气单胞菌（43%）最为常见，其次是温和气单胞菌（35%）和嗜水气单胞菌（20%）。嗜水气单胞菌在清洁的河流样本中含量较高，温和气单胞菌主要从死水采样区域分离得到，而豚鼠气单胞菌在海洋样本中分布更多。统计分析表明，气单胞菌的密度与各种理化参数的累积效应有关，而不是与单一因素有关。在气单胞菌属物种中，嗜水气单胞菌和温和气单胞菌具有高度溶血活性，而仅观察到11%的豚鼠气单胞菌能裂解绵羊红细胞。进行了乳鼠试验以阐明运动性气单胞菌的肠毒性，发现21%的受试菌株（一株豚鼠气单胞菌菌株）产生肠毒素。
