Caudwell A, Kuszala C
INRA, Station de Recherches sur les Mycoplasmes et les Arbovirus des Plantes, Dijon, France.
Res Microbiol. 1992 Oct;143(8):791-806. doi: 10.1016/0923-2508(92)90108-z.
We report here results concerning the best part of the grapevine to section, the best sampling period and an important adaptation of the extraction media for the diagnosis of a grapevine yellow disease by ELISA. The addition of Triton-X100, or better of Chaps enabled us to get clear results for the sensitive Vitis vinifera scion varieties. Until now, results have not been obtained for the symptomless root stock varieties. However, antigen concentration by molecular filtration may be a way to solve the problem of assaying root stocks.
我们在此报告关于葡萄藤用于检测的最佳部位、最佳采样时期以及用于通过酶联免疫吸附测定法（ELISA）诊断葡萄藤黄化病的提取介质的一项重要改进的结果。添加曲拉通-X100，或者更好的是添加两性离子去污剂3-[(3-胆酰胺丙基)二甲基铵]-1-丙磺酸（CHAPS），使我们能够针对敏感的酿酒葡萄接穗品种获得清晰的结果。到目前为止，对于无症状的砧木品种尚未得到相关结果。然而，通过分子过滤进行抗原浓缩可能是解决检测砧木问题的一种方法。
