Patten B E, Higgins P A, Whithear K G
Aust Vet J. 1984 May;61(5):151-5. doi: 10.1111/j.1751-0813.1984.tb07219.x.
An ELISA utilising a urease-antibody conjugate specific to chicken IgG was examined as an alternative to the serum agglutination and the haemagglutination inhibition tests in the diagnosis of Mycoplasma gallisepticum and M. synoviae infections in poultry. Use of a urease conjugate allowed the serum reactions to be appraised without the need for expensive photometric equipment. Non-specific binding of conjugate to antigen was eliminated by treatment of antigen coated microplates with 10% foetal calf serum in phosphate buffered saline. Some chicken serums produced non-specific reactions. These reactions were reduced without any loss of test sensitivity by making the initial 1:5 dilution of chicken serum in whole sheep serum rather than diluting buffer. Tests on serums from experimentally infected chickens showed that the urease ELISA was specific, and was as sensitive as the serum agglutination test but more sensitive than the haemagglutination inhibition test.
研究了一种利用对鸡免疫球蛋白G具有特异性的脲酶-抗体结合物的酶联免疫吸附测定（ELISA），作为血清凝集试验和血凝抑制试验的替代方法，用于诊断家禽的鸡败血支原体和滑液支原体感染。使用脲酶结合物可在无需昂贵的光度测定设备的情况下评估血清反应。通过用磷酸盐缓冲盐水中的10%胎牛血清处理包被抗原的微孔板，消除了结合物与抗原的非特异性结合。一些鸡血清产生非特异性反应。通过将鸡血清最初在全羊血清中进行1:5稀释而非在稀释缓冲液中稀释，这些反应减少了，且测试灵敏度没有任何损失。对实验感染鸡的血清进行的测试表明，脲酶ELISA具有特异性，与血清凝集试验一样灵敏，但比血凝抑制试验更灵敏。
