巯基与血红蛋白中血红素之间的相互作用。
Sulfhydryl groups and the interaction between the hemes in hemoglobin.
作者信息
RIGGS A F
出版信息
J Gen Physiol. 1952 May;36(1):1-16. doi: 10.1085/jgp.36.1.1.
Abstract
- Dialysis of a hemoglobin solution against slightly alkaline buffer (pH 8.68) causes a decrease in heme-heme interaction. The value of n in Hill's equation drops from 2.9 to 2.0, while the oxygen affinity increases slightly. Addition of glutathione largely reverses the effects of dialysis (n rises from 2.0 to 2.5 to 2.6). 2. Addition of the sulfhydryl reagent, p-chloromercuribenzoate (1.8 x 10(-3)M), results in a large though not complete blocking of heme-heme interaction. The value of n drops from 2.9 to 1.4. This effect is largely reversed with glutathione (n rises to 2.6).
摘要
- 用微碱性缓冲液（pH 8.68）对血红蛋白溶液进行透析会导致血红素-血红素相互作用减弱。希尔方程中的n值从2.9降至2.0，而氧亲和力略有增加。添加谷胱甘肽在很大程度上可逆转透析的影响（n值从2.0升至2.5再升至2.6）。2. 添加巯基试剂对氯汞苯甲酸（1.8×10⁻³M）会导致血红素-血红素相互作用受到很大但不完全的阻断。n值从2.9降至1.4。谷胱甘肽可在很大程度上逆转这种效应（n值升至2.6）。
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