Körmendy B
Central Veterinary Institute, Budapest, Hungary.
Acta Vet Hung. 1992;40(3):171-84.
On a 500-cow dairy farm a total of 866 young calves less than one month old were vaccinated with a heat-killed oil-adjuvated bacterin against Mycobacterium paratuberculosis over a period of five years. The vaccinated calves were tested by faecal microscopy, bacteriology and serology on the day of vaccination, at the age of 3, 6, 9 and 12 months, at breeding age, and on the day of calving. A total of 721 bull calves and 379 female calves served as unvaccinated controls in two groups. The results were evaluated by trend analyses. Vaccination greatly reduced the faecal shedding of mycobacteria as demonstrated by the annual faecal microscopic examinations. During the last 6 months of the experiment only 9 of 612 samples were found positive by microscopy and by bacterial culture. The number of seropositive animals and the antibody titres demonstrated by the complement fixation test (CFT) and agar gel immunodiffusion (AGID) increased during the first three years. Later on, both the number of seropositive animals and CFT titres decreased.
在一个拥有500头奶牛的奶牛场，在五年时间里，共有866头不到1月龄的小牛犊接种了针对副结核分枝杆菌的热灭活油佐剂菌苗。对接种的小牛犊在接种当天、3、6、9和12月龄、配种年龄以及产犊当天进行粪便显微镜检查、细菌学和血清学检测。总共721头公牛犊和379头母牛犊作为两组未接种疫苗的对照。通过趋势分析对结果进行评估。年度粪便显微镜检查表明，接种疫苗大大减少了分枝杆菌的粪便排出量。在实验的最后6个月，612份样本中只有9份通过显微镜检查和细菌培养呈阳性。通过补体结合试验（CFT）和琼脂凝胶免疫扩散试验（AGID）检测的血清阳性动物数量和抗体滴度在最初三年有所增加。后来，血清阳性动物数量和CFT滴度均下降。
