Spangler E, Heider L E, Bech-Nielsen S, Dorn C R
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Am J Vet Res. 1991 Aug;52(8):1197-200.
The purpose of this study was to document the effect of calfhood vaccination for Mycobacterium paratuberculosis on a serologic ELISA. Fifteen calves vaccinated with a killed paratuberculosis vaccine and 5 unvaccinated control calves were tested from the first through the fifteenth month of life. Age of vaccination ranged from 5 to 40 days. Blood samples were collected prior to vaccination and periodically thereafter. Serum antibody was analyzed by use of the ELISA. All calves were ELISA-negative prior to vaccination. Thirteen of 15 vaccinated calves became ELISA-positive between 2 and 6 months after vaccination. The unvaccinated cohort remained ELISA-negative. Wide-spread use of vaccine may interfere with diagnosis of paratuberculosis and with control programs that are based on serologic tests that measure humoral antibody.
本研究的目的是记录犊牛期接种副结核分枝杆菌疫苗对血清学酶联免疫吸附测定（ELISA）的影响。对15头接种了副结核灭活疫苗的犊牛和5头未接种疫苗的对照犊牛从出生后的第1个月到第15个月进行了检测。接种疫苗的年龄范围为5至40天。在接种疫苗前和之后定期采集血样。使用ELISA分析血清抗体。所有犊牛在接种疫苗前ELISA检测均为阴性。15头接种疫苗的犊牛中有13头在接种疫苗后2至6个月内ELISA检测变为阳性。未接种疫苗的一组犊牛ELISA检测仍为阴性。疫苗的广泛使用可能会干扰副结核病的诊断以及基于检测体液抗体的血清学检测的控制程序。
