Farkas T, Drén C, Németh I, Dobos-Kovács M, Povazsán J, Sághy E
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
Acta Vet Hung. 1992;40(3):207-23.
Chicken anaemia virus (CAV) infection was demonstrated, by both serology and virus isolation, in 1- to 6-week-old broiler chickens originated from various parent flocks in Hungary. Total losses in the broiler flocks were estimated at 7 to 8% and about 25% of the chickens failed to reach target body mass by the 7th week of life. The clinical signs, postmortem lesions and histopathological changes of the affected chickens were similar to those of naturally occurring CAV-induced infectious anaemia of young chickens. In MDCC-MSB1 cell cultures, a chloroform-resistant virus smaller than 50 nm in diameter, resistant to heating at 70 degrees C for 30 min, and antigenically very closely related to the Cux-1 strain of CAV was isolated from the liver of naturally diseased broilers. This virus isolate was designated the Bia strain of CAV. Inoculation of susceptible 1-day-old SPF chicks with a CAV-positive liver extract from naturally diseased broilers caused pathological changes characteristic of CAV infection, namely impaired growth, severe anaemia with atrophy of the bone marrow, marked atrophy of the lymphoid organs and petechial haemorrhages throughout the body. A quite similar pathological syndrome was also induced by inoculation of 1-day-old SPF chicks with the MDCC-MSB1 cell-culture-propagated new Bia strain of CAV. The CAV was successfully reisolated from the livers of experimentally inoculated birds, and antibodies to the reference Cux-1 strain of CAV were also demonstrated by the indirect immunofluorescence test in sera of naturally diseased and experimentally inoculated chickens. No antibodies were found against infectious bursal disease virus, reticuloendotheliosis virus, Marek's disease herpesvirus as well as avian adenoviruses and reoviruses. The reported disease of young broiler chickens was associated with natural infection of a new isolate of CAV. On the basis of its physicochemical, antigenic and pathogenic characteristics, this virus is similar to other strains of CAV isolated from chickens in other countries.
通过血清学和病毒分离方法，在匈牙利来自不同亲本鸡群的1至6周龄肉鸡中证实了鸡贫血病毒（CAV）感染。肉鸡群的总损失估计为7%至8%，约25%的鸡在7周龄时未能达到目标体重。患病鸡的临床症状、死后病变和组织病理学变化与自然发生的CAV诱导的雏鸡感染性贫血相似。在MDCC-MSB1细胞培养物中，从自然患病肉鸡的肝脏中分离出一种直径小于50 nm、耐氯仿、70℃加热30分钟仍存活且抗原性与CAV的Cux-1株密切相关的病毒。该病毒分离株被命名为CAV的Bia株。用来自自然患病肉鸡的CAV阳性肝脏提取物接种易感的1日龄SPF雏鸡，引起了CAV感染的特征性病理变化，即生长受损、严重贫血伴骨髓萎缩、淋巴器官明显萎缩和全身瘀点性出血。用MDCC-MSB1细胞培养增殖的新CAV Bia株接种1日龄SPF雏鸡也诱导出了非常相似的病理综合征。从实验接种鸡的肝脏中成功地再次分离出了CAV，并且通过间接免疫荧光试验在自然患病和实验接种鸡的血清中也检测到了针对CAV参考Cux-1株的抗体。未发现针对传染性法氏囊病病毒、网状内皮组织增殖病病毒、马立克氏病疱疹病毒以及禽腺病毒和呼肠孤病毒的抗体。报道的雏肉鸡疾病与一种新的CAV分离株的自然感染有关。基于其理化、抗原和致病特性，该病毒与在其他国家从鸡中分离出的其他CAV株相似。
