McNulty M S, Connor T J, McNeilly F, Spackman D
Veterinary Research Laboratories, Stormont, Belfast, Northern, Ireland.
Avian Dis. 1989 Oct-Dec;33(4):691-4.
Chicken anemia agent (CAA) was isolated from broiler chickens in Texas with a blue wing or anemia dermatitis-like syndrome. Specific-pathogen-free chicks inoculated with field material developed anemia, and CAA was isolated in MDCC-MSB1 cells from bone marrow and lymphoid tissue from inoculated chicks. One isolate, designated EF88/78/276, was further characterized. Infectivity of EF88/78/276 was resistant to treatment with chloroform and with heat at 70 C for 5 minutes. EF88/78/276 was indistinguishable from the Cux-1 and Gifu-1 isolates of CAA by cross-neutralization tests. Almost all 1-day-old susceptible chicks inoculated intramuscularly with EF88/78/276 developed anemia, but contact-infected chicks did not. Antibody to CAA was detected in broiler breeder flocks from Texas, the Delmarva peninsula, and Alabama.
鸡贫血因子（CAA）是从得克萨斯州患有蓝翅或贫血性皮炎样综合征的肉鸡中分离出来的。用田间材料接种无特定病原体的雏鸡会出现贫血症状，并且从接种雏鸡的骨髓和淋巴组织中，在MDCC - MSB1细胞中分离出了CAA。对其中一个分离株，命名为EF88/78/276，进行了进一步的特性分析。EF88/78/276的感染性对氯仿处理以及70℃加热5分钟具有抗性。通过交叉中和试验，EF88/78/276与CAA的Cux - 1和岐阜 - 1分离株无法区分。几乎所有经肌肉注射EF88/78/276的1日龄易感雏鸡都会出现贫血，但接触感染的雏鸡则不会。在来自得克萨斯州、德尔马瓦半岛和阿拉巴马州的肉种鸡群中检测到了CAA抗体。
