Terabe S
Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
J Pharm Biomed Anal. 1992 Oct-Dec;10(10-12):705-15. doi: 10.1016/0731-7085(91)80069-l.
Micellar electrokinetic chromatography (MEKC) permits the separation of electrically neutral analytes by chromatographic principles in a capillary electrophoresis system. The most effective way to obtain high resolution in MEKC is to increase the separation factor, as in conventional chromatography. The separation factor in MEKC depends on the molecular structure of the micelle and hence on the surfactant used, the pH of solution, and the nature of any additives to the micellar solution. The hydrophilic moieties of surfactant molecules generally affect selectivity more than do the hydrophobic moieties. Chiral surfactants enable the enantiomeric separation of mixtures of chiral solutes to be achieved. Mixed micelles consisting of ionic and nonionic surfactants display different selectivity from that of single ionic micelles. Additives such as cyclodextrins, ion-pair reagents, urea, organic solvents and metals can also serve as useful modifiers of the micellar solution for improving separation. In particular, cyclodextrins are useful for the separation of aromatic isomers and enantiomers. A general introductory guide to the design of successful separations by MEKC is proposed, based primarily on the author's work.
胶束电动色谱法（MEKC）可在毛细管电泳系统中依据色谱原理分离电中性分析物。与传统色谱法一样，在MEKC中获得高分辨率的最有效方法是提高分离因子。MEKC中的分离因子取决于胶束的分子结构，因此取决于所用的表面活性剂、溶液的pH值以及胶束溶液中任何添加剂的性质。表面活性剂分子的亲水部分通常比疏水部分对选择性的影响更大。手性表面活性剂能够实现手性溶质混合物的对映体分离。由离子型和非离子型表面活性剂组成的混合胶束表现出与单一离子型胶束不同的选择性。诸如环糊精、离子对试剂、尿素、有机溶剂和金属等添加剂也可用作胶束溶液的有效改性剂以改善分离效果。特别是，环糊精对于芳香异构体和对映体的分离很有用。本文主要基于作者的工作，提出了一份关于通过MEKC成功进行分离设计的通用入门指南。
