Terabe Shigeru
Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Hyogo 678-1297, Japan.
Chem Rec. 2008;8(5):291-301. doi: 10.1002/tcr.20156.
Capillary electrophoresis (CE) is a relatively new method of analytical separation having the advantages of high separation efficiency, requirement of a small sample amount, low operating cost, and fast separation time. CE is a separation method where the analyte migrates under an electric field due to a charge on the analyte. Hence, CE was unable to separate neutral analytes until the advent of micellar electrokinetic chromatography (MEKC). MEKC is performed with an addition of ionic micelles to an electrophoretic medium, where a portion of the analyte is incorporated into the micelle and has an apparent charge, which can be subject to electrophoretic separation. The migration velocity of the neutral analyte in MEKC depends on what portion of the analyte is incorporated into the micelle. Thus, the separation principle of MEKC is similar to that of chromatography, although the micelle corresponding to the stationary phase in chromatography is not stationary inside the capillary. The fundamental characteristics and theoretical treatments of the behavior of the analyte in MEKC were studied extensively by the author's group. MEKC has been established as one of the most popular separation modes in CE. This review describes how MEKC was developed and how it is useful as a method of analytical separation.
毛细管电泳（CE）是一种相对较新的分析分离方法，具有分离效率高、样品用量少、操作成本低和分离时间快等优点。CE是一种分离方法，其中分析物由于其电荷而在电场下迁移。因此，在胶束电动色谱（MEKC）出现之前，CE无法分离中性分析物。MEKC是通过向电泳介质中添加离子胶束来进行的，其中一部分分析物被纳入胶束并具有表观电荷，从而可以进行电泳分离。中性分析物在MEKC中的迁移速度取决于分析物被纳入胶束的比例。因此，MEKC的分离原理与色谱法相似，尽管在色谱法中对应于固定相的胶束在毛细管内并非固定不动。作者所在的研究小组对分析物在MEKC中的行为的基本特征和理论处理进行了广泛研究。MEKC已成为CE中最流行的分离模式之一。本综述描述了MEKC是如何发展的以及它作为一种分析分离方法的实用性。
