药物制剂中咪唑类抗真菌药物的高效液相色谱分析

HPLC analysis of imidazole antimycotic drugs in pharmaceutical formulations.

作者信息

Di Pietra A M, Cavrini V, Andrisano V, Gatti R

机构信息

Department of Pharmaceutical Sciences, University of Bologna, Italy.

出版信息

J Pharm Biomed Anal. 1992 Oct-Dec;10(10-12):873-9. doi: 10.1016/0731-7085(91)80094-p.

DOI:10.1016/0731-7085(91)80094-p

PMID:1298396

Abstract

Reversed-phase HPLC on different column packing materials (Hypersil C-18, Spherisorb-CN, Chromspher-B) is used to obtain selective separations of imidazole antimycotic drugs, such as ketoconazole, clotrimazole, tioconazole, bifonazole, isoconazole, econazole, miconazole and fenticonazole. The use of a post-column on-line photochemical reactor is shown to be useful for the enhancement of the sensitivity of the HPLC analysis with UV detection. The proposed HPLC methods are applied to the analysis of commercial dosage forms (creams) with solid-phase extraction (SPE) procedure, using a diol sorbent, being found to be a convenient technique for the sample preparation giving quantitative drug recovery.

摘要

采用不同的柱填充材料（Hypersil C-18、Spherisorb-CN、Chromspher-B）进行反相高效液相色谱法，以实现对咪唑类抗真菌药物（如酮康唑、克霉唑、噻康唑、联苯苄唑、异康唑、益康唑、咪康唑和芬替康唑）的选择性分离。结果表明，使用柱后在线光化学反应器有助于提高高效液相色谱法紫外检测的灵敏度。所提出的高效液相色谱法应用于商业剂型（乳膏）的分析，采用二醇吸附剂的固相萃取（SPE）程序，发现这是一种方便的样品制备技术，可实现药物的定量回收。