Hanks C T, Wataha J C, Parsell R R, Strawn S E
University of Michigan School of Dentistry, Ann Arbor.
J Endod. 1992 Dec;18(12):589-96. doi: 10.1016/S0099-2399(06)81328-2.
Until adhesiveness of dentin bonding agents and other restorative materials to dental structures can be assured, microleakage into resulting "gaps" and dentin permeability will remain major concerns in cases of pulpal irritation. The objectives of the present study were to (a) delineate the kinds and levels of metabolic cytotoxicity of the GLUMA and Scotchbond 2 systems as well as glutaraldehyde and 2-hydroxyethylmethacrylate, and (b) compare the effects of these same materials after diffusion through dentin discs approximately 0.5-mm thick. In monolayer cultures, glutaraldehyde was much more cytotoxic than 2-hydroxyethylmethacrylate. However, GLUMA sealer and Scotchbond 2 adhesive exhibited similar cytotoxicity in monolayer cultures. After diffusion through dentin, glutaraldehyde and 2-hydroxyethylmethacrylate effects were diluted 14.7 and 26.7 times, respectively. The postdiffusional effects of the GLUMA and Scotchbond 2 systems were not significantly different and less than those effects in monolayer cultures. This study should help in the evaluation of possible causes of pulpal irritation following restorative procedures.
在确保牙本质粘结剂和其他修复材料与牙体结构的粘结性之前，微渗漏进入形成的“间隙”以及牙本质渗透性在牙髓刺激病例中仍将是主要问题。本研究的目的是：（a）描述GLUMA和Scotchbond 2系统以及戊二醛和甲基丙烯酸2-羟乙酯的代谢细胞毒性的种类和水平；（b）比较这些相同材料在通过约0.5毫米厚的牙本质盘扩散后的效果。在单层培养中，戊二醛的细胞毒性比甲基丙烯酸2-羟乙酯大得多。然而，GLUMA封闭剂和Scotchbond 2粘结剂在单层培养中表现出相似的细胞毒性。通过牙本质扩散后，戊二醛和甲基丙烯酸2-羟乙酯的作用分别被稀释了14.7倍和26.7倍。GLUMA和Scotchbond 2系统的扩散后作用没有显著差异，且小于单层培养中的作用。本研究应有助于评估修复术后牙髓刺激的可能原因。
