Senkevich T G, Muravnik G L, Chernos V I
Mol Gen Mikrobiol Virusol. 1992 Sep-Oct(9-10):13-5.
In order to identify ectromelia virus (EMV) genome regions which may contain genes responsible for the specific pathogenicity of this virus, blot cross-hybridization of EMV DNA with those of other orthopoxviruses was performed. Two hybridization schemes were employed: one of them included hybridization of labelled cloned fragments of EMV with digests of other viral DNAs, the other, reciprocal, consisted in hybridization of labelled total DNAs of various orthopoxviruses with digests of the region of EMV DNA adjacent to the right-terminal inverted repeat. It was demonstrated that the counterpart to an approximately 8-kilobase pair portion of EMV genome flanking the inverted repeat could be detected only in the cowpox virus genome but not in the genomes of vaccinia and rabbitpox viruses. XhoI-O and XhoI-K fragments of EMV DNA contained, along with genes found in other poxviruses, certain genes which appeared to be unique for EMV. It is postulated that some of these genes may determine the specific biological properties of EMV, including its pathogenicity for mice.
为了鉴定可能包含负责该病毒特异性致病性基因的痘苗病毒（EMV）基因组区域，进行了EMV DNA与其他正痘病毒DNA的印迹交叉杂交。采用了两种杂交方案：其中一种包括用其他病毒DNA的酶切产物与标记的EMV克隆片段进行杂交，另一种反向杂交方案是用各种正痘病毒的标记总DNA与EMV DNA中与右末端反向重复序列相邻区域的酶切产物进行杂交。结果表明，仅在牛痘病毒基因组中能检测到与EMV基因组中反向重复序列侧翼约8千碱基对部分相对应的序列，而在痘苗病毒和兔痘病毒基因组中未检测到。EMV DNA的XhoI-O和XhoI-K片段除了含有在其他痘病毒中发现的基因外，还含有一些似乎是EMV特有的基因。据推测，其中一些基因可能决定EMV的特定生物学特性，包括其对小鼠的致病性。
