Shchelkunov S N, Safronov P F, Totmenin A V, Petrov N A, Ryazankina O I, Gutorov V V, Kotwal G J
Department of Molecular Biology of Genomes, State Research Center of Virology and Biotechnology Vector Koitsovo, Novosibirsk Region, Russia.
Virology. 1998 Apr 10;243(2):432-60. doi: 10.1006/viro.1998.9039.
Sequencing and computer analysis of the left (52,283 bp) and right (49,649 bp) variable DNA regions of the cowpox virus strain GRI-90 (CPV-GRI) has revealed 51 and 37 potential open reading frames (ORFs), respectively. Comparison of the structure-function organization of these DNA regions of CPV-GRI with those previously published for corresponding regions of genomes of vaccinia virus, strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR); and variola major virus, strains India-1967 (VAR-IND), Bangladesh-1975 (VAR-BSH); and alastrim variola minor virus, strain Garcia-1966 (VAR-GAR), was performed. Within the left terminal region under study, an extended DNA sequence (14,171 bp), unique to CPV, has been found. Within the right region of the CPV-GRI genome two segments, which are unique to CPV DNA (1579 and 3585 bp) have been found. Numerous differences have been revealed in the genetic structure of CPV-GRI DNA regions, homologous to fragments of the genomes of the above-mentioned orthopoxvirus strains. A cluster of ORFs with structural similarity ot immunomodulatory and host range function of other poxviruses have also been detected. A comparison of the sequences of ORF B, crmA, crmB, crmC, IMP, and CHO hr genes of CPV Brighton strain (CPV-BRI) with the corresponding genes in strain GRI-90 have revealed an identity at the amino acid level ranging from 82 to 96% between the two strains. The findings are significant in light of the recent demonstration of CPV as an important poxvirus model system to probe the precise in vivo role(s) of the unique virally encoded immunomodulatory proteins. Also, the presence of a complete and intact repertoire of immunomodulatory proteins, ring canal proteins family, and host range genes indicates that CPV may have been the most ancient of all studied orthopoxviruses.
对牛痘病毒株GRI - 90（CPV - GRI）的左侧（52,283 bp）和右侧（49,649 bp）可变DNA区域进行测序和计算机分析，分别揭示了51个和37个潜在开放阅读框（ORF）。将CPV - GRI这些DNA区域的结构 - 功能组织与先前发表的痘苗病毒株哥本哈根（VAC - COP）和西储（VAC - WR）；以及天花病毒株印度 - 1967（VAR - IND）、孟加拉国 - 1975（VAR - BSH）；和类天花病毒株加西亚 - 1966（VAR - GAR）基因组相应区域的结构 - 功能组织进行了比较。在所研究的左侧末端区域内，发现了一段CPV特有的延伸DNA序列（14,171 bp）。在CPV - GRI基因组的右侧区域，发现了两段CPV DNA特有的片段（1579和3585 bp）。在与上述正痘病毒株基因组片段同源的CPV - GRI DNA区域的遗传结构中揭示了许多差异。还检测到一组与其他痘病毒免疫调节和宿主范围功能具有结构相似性的ORF。将CPV布莱顿株（CPV - BRI）的ORF B、crmA、crmB、crmC、IMP和CHO hr基因序列与GRI - 90株中的相应基因进行比较，发现两株之间氨基酸水平的同一性在82%至96%之间。鉴于最近证明CPV是一种重要的痘病毒模型系统，可用于探究独特的病毒编码免疫调节蛋白在体内的确切作用，这些发现具有重要意义。此外，免疫调节蛋白、环形管道蛋白家族和宿主范围基因的完整和完好的全套存在表明CPV可能是所有研究过的正痘病毒中最古老的。
