Senkevich T G, Vovk T S, Antonova T P, Chernos V I
Mol Gen Mikrobiol Virusol. 1986 Dec(12):26-9.
Four DNA-temperature-sensitive (ts) mutations were mapped in the genome of vaccinia virus (VV). Physical mapping of these mutations was performed by restriction analysis of the genomes of recombinants between VV DNA- ts mutants and ectromelia virus as well as by the marker rescue with cloned restriction fragments of VV DNA. One of the mutations was mapped on the HindIII-E-fragment. Biochemical studies of this mutant indicate that the mutation is not in the DNA polymerase gene which is located on the same fragment. The other three mutations were mapped in a 10 kilobase region in the middle of the HindIII-D-fragment. As shown previously, these mutations inactivate different genes, and the products of these genes participate directly in the DNA synthesis. Thus, at least three proteins involved in the VV DNA synthesis are encoded by neighboring genes in the central part of the viral genome.
在痘苗病毒（VV）基因组中定位了四个对温度敏感（ts）的DNA突变。通过对VV DNA-ts突变体与埃可病毒之间重组体的基因组进行限制性分析，以及用VV DNA的克隆限制性片段进行标记拯救，对这些突变进行了物理定位。其中一个突变位于HindIII-E片段上。对该突变体的生化研究表明，该突变不在位于同一片段上的DNA聚合酶基因中。另外三个突变位于HindIII-D片段中部的一个10千碱基区域内。如前所示，这些突变使不同的基因失活，并且这些基因的产物直接参与DNA合成。因此，病毒基因组中部相邻基因编码了至少三种参与VV DNA合成的蛋白质。
