Opartkiattikul N, Funahara Y, Hijikata-Okunomiya A, Yamaguchi N, Talalak P
Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1992;23 Suppl 2:47-51.
We asked the question, "Can thalassemic erythrocytes play some role in alteration of the hemostatic system?", because clinical examination of thalassemic patients shows symptoms and signs related to alterations in hemostatic and circulatory systems, and thalassemic erythrocytes are different from normal erythrocytes. We obtained one of the answers to the question: The erythrocytes of postsplenectomized patients of beta-thalassemia/HbE disease could stimulate their own platelets to aggregate spontaneously. To know the role of erythrocytes in platelet aggregation, we wanted to examine the effect of thalassemic erythrocytes on the coagulation system by focusing of PF3-like activity of erythrocytes, because PF3-like activity of the ghosts of erythrocytes had been reported. For the study, we tried to develop a technique that was accurate and sensitive enough to detect PF3-like activity of blood. The system we developed was the following: 1) We activated the intrinsic coagulation pathway of commercial standard plasma by ellagic acid. 2) CaCl2, a fixed amount of PF 3 and synthetic thrombin inhibitor MD 805 were added to the reaction mixture. 3) At a fixed time, thrombin activity in the mixture was measured by using S-2238 as a substrate. At full activation of the contact system by ellagic acid, the amount of thrombin formed in a certain time depended on the amount of PF3-like substances such as cephalin, freeze-thawed platelets or ghosts of erythrocytes added to the test system, indicating that PF3-like activity of those substances can be measured by the activity of thrombin generated in a fixed time.(ABSTRACT TRUNCATED AT 250 WORDS)
“地中海贫血红细胞能否在止血系统改变中发挥某种作用？”，因为对地中海贫血患者的临床检查显示出与止血和循环系统改变相关的症状和体征，而且地中海贫血红细胞与正常红细胞不同。我们得到了这个问题的一个答案：β-地中海贫血/HbE病脾切除术后患者的红细胞能够刺激自身血小板自发聚集。为了了解红细胞在血小板聚集中的作用，我们想通过关注红细胞的PF3样活性来研究地中海贫血红细胞对凝血系统的影响，因为已有报道称红细胞膜空壳具有PF3样活性。为了进行这项研究，我们试图开发一种技术，该技术要足够准确和灵敏，以检测血液中的PF3样活性。我们开发的系统如下：1）我们用鞣花酸激活商业标准血浆的内源性凝血途径。2）向反应混合物中加入氯化钙、固定量的PF3和合成凝血酶抑制剂MD 805。3）在固定时间，以S-2238为底物测量混合物中的凝血酶活性。当鞣花酸使接触系统完全激活时，在一定时间内形成的凝血酶量取决于加入测试系统中的PF3样物质（如脑磷脂、冻融血小板或红细胞膜空壳）的量，这表明这些物质的PF3样活性可以通过固定时间内产生的凝血酶活性来测量。（摘要截短于250字）
