Timan I S, Funahara Y, Setiabudy R, Latu J, Silman E
Faculty of Medicine, University of Indonesia, Jakarta.
Southeast Asian J Trop Med Public Health. 1993;24 Suppl 1:216-8.
Platelet factor 3 (PF3) is a platelet membrane component that plays an important role in the activation of the coagulation mechanism. Whenever platelet activation occurred, PF3 is released and participates in thrombin formation. Erythrocyte membrane fraction has also some PF3 like activity, and in abnormal erythrocyte membrane disorders, eg thalassemia, some of the membrane fraction accelerates platelet activation by increasing the PF3 activity. Formerly it was difficult to measure the PF3 activity in plasma. Recently a sensitive chromogenic test to determine the PF3 activity, which could detect the changes in PF3 activity with time, was introduced. This study was done to observe the effect of abnormal erythrocyte on platelet activation. The results obtained using the chromogenic method are the following: whole blood taken from normal subjects showed OD 0.11 +/- 0.06 at 0 minutes after blood collection and then increased significantly (p < 0.01) to 0.21 +/- 0.10 after 90 minutes, while the platelet count did not differ significantly (p > 0.05). Those results showed that there were some platelet activation after 90 minutes as seen by the increased PF3 activity, with no significant change in platelet counts. In beta-thalassemic trait subjects the PF3 activity in whole blood at 0 minutes did not differ significantly compared to the normal subjects, but after 90 minutes it was significantly higher (p < 0.01), OD 0.52 +/- 0.35. However the PF3 in platelet rich plasma at 90 minutes did not increase. The platelet count after 90 minutes was significantly decreased (p < 0.01) This result suggest that the increase in PF3 activity was caused by the role of the abnormal erythrocytes.
血小板因子3（PF3）是一种血小板膜成分，在凝血机制的激活中起重要作用。每当血小板激活发生时，PF3就会释放并参与凝血酶的形成。红细胞膜部分也具有一些类似PF3的活性，在异常红细胞膜疾病（如地中海贫血）中，部分膜成分通过增加PF3活性来加速血小板激活。以前很难测量血浆中的PF3活性。最近引入了一种灵敏的显色试验来测定PF3活性，该试验可以检测PF3活性随时间的变化。本研究旨在观察异常红细胞对血小板激活的影响。使用显色法获得的结果如下：从正常受试者采集的全血在采血后0分钟时OD值为0.11±0.06，然后在90分钟后显著增加（p<0.01）至0.21±0.10，而血小板计数无显著差异（p>0.05）。这些结果表明，90分钟后可见一些血小板激活，表现为PF3活性增加，而血小板计数无显著变化。在β地中海贫血特征受试者中，全血中0分钟时的PF3活性与正常受试者相比无显著差异，但90分钟后显著更高（p<0.01），OD值为0.52±0.35。然而，富含血小板血浆在90分钟时的PF3并未增加。90分钟后的血小板计数显著降低（p<0.01）。该结果表明，PF3活性的增加是由异常红细胞的作用引起的。
