Soncul H, Ersöz A, Gökgöz L, Karasu C, Ayrancioglu K, Sinci V, Altan M
Department of Thoracic and Cardiovascular Surgery, Gazi University Medical School, Ankara, Turkey.
Jpn Heart J. 1992 Nov;33(6):843-50. doi: 10.1536/ihj.33.843.
In order to determine the effect of adenosine triphosphate (ATP) and adenosine in cardioplegic solutions, a comparative study has been undertaken in isolated guinea pig hearts using the Langendorff perfusion technique as a model of cardiopulmonary bypass. The hearts (n = 10 in each group) previously being perfused by Krebs-Henseleit solution, were arrested by one of the following cardioplegic solutions: 1) Potassium 20 mM/L (Plegisol), 2) Potassium 20 mM/L+ATP 10 mM/L, 3) Adenosine 10 mM/L, 4) Adenosine 10 mM/L+ATP 10 mM/L. After 45 min of hypothermic ischemia, postischemic recovery of heart rate, ventricular contractility, heart work and postischemic changes in tissue enzymes (LDH, SGOT, SGPT) were compared among the 4 different cardioplegic solutions. Arrest time and number of arrest beats were also recorded and compared among the groups. Although similar beneficial results on postischemic recovery were achieved with adenosine cardioplegia and with ATP supplemented potassium cardioplegia, ATP supplemented adenosine cardioplegia did not show any beneficial effects on postischemic recovery.
为了确定三磷酸腺苷（ATP）和腺苷在心脏停搏液中的作用，采用Langendorff灌注技术作为体外循环模型，对豚鼠离体心脏进行了一项对比研究。心脏（每组n = 10）先前用Krebs-Henseleit溶液灌注，然后用以下心脏停搏液之一使其停搏：1）20 mM/L钾（普列吉索尔），2）20 mM/L钾+10 mM/L ATP，3）10 mM/L腺苷，4）10 mM/L腺苷+10 mM/L ATP。在低温缺血45分钟后，比较了4种不同心脏停搏液之间心率、心室收缩力、心脏作功的缺血后恢复情况以及组织酶（乳酸脱氢酶、谷草转氨酶、谷丙转氨酶）的缺血后变化。还记录并比较了各组的停搏时间和停搏搏动次数。尽管腺苷心脏停搏和补充ATP的钾心脏停搏在缺血后恢复方面取得了相似的有益结果，但补充ATP的腺苷心脏停搏对缺血后恢复未显示出任何有益作用。
