对在巴氏链霉菌和变铅青链霉菌种间交配后分离得到的线性染色体外DNA元件（pBL1）的表征。

Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans.

作者信息

Zotchev S B, Soldatova L I, Orekhov A V, Schrempf H

机构信息

Institute of Genetics, Moscow, Russia.

出版信息

Res Microbiol. 1992 Nov-Dec;143(9):839-45. doi: 10.1016/0923-2508(92)90071-u.

DOI:10.1016/0923-2508(92)90071-u

PMID:1299837

Abstract

Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3'- and 5'-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.

摘要

班贝格链霉菌S712含有一个640 kb的巨大线性质粒PSB1。与无质粒的淡紫链霉菌菌株TK64接合后，鉴定出携带一个较小的染色体外DNA元件pBL1的接合子。pBL1是一个43 kb的线性DNA分子，与一种蛋白质结合，该蛋白质可保护它免受3'-和5'-核酸外切酶的攻击。这种蛋白质的缺失会大幅降低pBL1的转化效率。pBL1与班贝格链霉菌菌株的线性质粒和染色体DNA具有同源性。