灰色链霉菌线性染色体的物理图谱。
Physical map of the linear chromosome of Streptomyces griseus.
作者信息
Lezhava A, Mizukami T, Kajitani T, Kameoka D, Redenbach M, Shinkawa H, Nimi O, Kinashi H
机构信息
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
出版信息
J Bacteriol. 1995 Nov;177(22):6492-8. doi: 10.1128/jb.177.22.6492-6498.1995.
Abstract
The chromosomal DNA of Streptomyces griseus 2247 (a derivative of strain IFO3237) was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestion with AseI and DraI gave 15 and 9 fragments, respectively, the total sizes of which were 7.8 Mb. All the AseI and DraI fragments were aligned on a linear chromosome map by using linking plasmids and cosmids. PFGE analysis of the intact chromosome also showed a linear DNA band of about 8 Mb. Detailed physical maps of both terminal regions were constructed; they revealed the presence of a 24-kb terminal inverted repeat on each end. PFGE analysis with and without proteinase K treatment suggested that each end of the chromosome carries a protein molecule.
摘要
用几种限制性内切酶消化灰色链霉菌2247（IFO3237菌株的衍生物）的染色体DNA，并通过脉冲场凝胶电泳（PFGE）进行分析。用AseI和DraI消化分别产生15个和9个片段，其总大小为7.8 Mb。通过使用连接质粒和黏粒，将所有AseI和DraI片段排列在一条线性染色体图谱上。完整染色体的PFGE分析也显示出一条约8 Mb的线性DNA带。构建了两个末端区域的详细物理图谱；它们揭示了两端各存在一个24 kb的末端反向重复序列。有蛋白酶K处理和无蛋白酶K处理的PFGE分析表明，染色体的每一端都携带一个蛋白质分子。
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