Gabbay S, Rosenberg M, Warburg M R, Rott R, Katz U
Department of Biology Technion, Israel Institute of Technology, Haifa.
Biol Cell. 1992;76(2):185-91. doi: 10.1016/0248-4900(92)90211-i.
Changes in characteristic components of the skin epidermis of the large tadpole of Pelobates syriacus were studied throughout its development. The fate of two specific cells in the skin epidermis was followed, from the young tadpole to the adult was studied. It was found that flask-shaped type cells in the tadpole epidermis which are PAS-positive, stain with peanut lectin (PNA). There is no detectable band 3 in the premetamorphosed stages, and mitochondria-rich cells are very rare. This pattern of staining changes completely upon metamorphosis: the PAS-positive cells, specific to the tadpole epidermis disappear, and the mitochondria-rich (MR) cells in the adult skin epithelium react with polyclonal anti-band 3 antibody. Western blot analysis showed the presence of a band 3-like protein of about 95 kDa, only in the adult epithelial extract, corroborating the immunocytochemical observations. The finding of the presence of band 3-like protein in the MR cells of Pelobates, is similar to the observations made in the skin of other amphibian species. On the other hand, the binding of peanut lectin to MR cells is species-specific, since it does not react with the MR cells in the skin epithelium of Pelobates syriacus.
研究了叙利亚蟾蜍大蝌蚪皮肤表皮特征成分在其整个发育过程中的变化。追踪了皮肤表皮中两种特定细胞从幼蝌蚪到成体的命运。发现蝌蚪表皮中呈PAS阳性的烧瓶状细胞能与花生凝集素（PNA）结合。在变态前阶段未检测到带3，富含线粒体的细胞也非常罕见。变态时这种染色模式完全改变：蝌蚪表皮特有的PAS阳性细胞消失，成体皮肤上皮中富含线粒体的（MR）细胞与多克隆抗带3抗体发生反应。蛋白质免疫印迹分析表明，仅在成体上皮提取物中存在一条约95 kDa的类似带3的蛋白质条带，这证实了免疫细胞化学观察结果。在叙利亚蟾蜍的MR细胞中发现类似带3的蛋白质，这与在其他两栖类物种皮肤中的观察结果相似。另一方面，花生凝集素与MR细胞的结合具有物种特异性，因为它不与叙利亚蟾蜍皮肤上皮中的MR细胞发生反应。
