Kamphuis W, Monyer H, De Rijk T C, Lopes da Silva F H
Department of Experimental Zoology, University of Amsterdam, The Netherlands.
Neurosci Lett. 1992 Dec 14;148(1-2):51-4. doi: 10.1016/0304-3940(92)90802-e.
The level of the mRNAs encoding the AMPA-selective glutamate receptors-A and -B, alternatively splice variants, Flip and Flop, was studied by in situ hybridization in the brains of rats kindled by Schaffer collateral/commissural-fiber stimulation. In comparison to control animals, the expression level of the Flip variant of both GluR-A and GluR-B mRNAs was bilaterally enhanced in the dentate granule neurons of kindled animals 24 h after last-generalized seizure, whereas no obvious alterations were observed in the GluR-A Flop and GluR-B Flop mRNA variants. In kindled animals, studied 1 month after the last seizure, GluR-A Flip and GluR-B Flip mRNA had returned to control levels. We suggest that these changes may result in an enhanced glutamate receptor sensitivity in the fascia dentata during kindling.
通过原位杂交技术，研究了由Schaffer侧支/联合纤维刺激点燃的大鼠大脑中，编码AMPA选择性谷氨酸受体-A和-B（可变剪接变体Flip和Flop）的mRNA水平。与对照动物相比，在最后一次全身性癫痫发作24小时后，点燃动物的齿状颗粒神经元中，GluR-A和GluR-B mRNA的Flip变体的表达水平双侧增强，而GluR-A Flop和GluR-B Flop mRNA变体未观察到明显变化。在最后一次癫痫发作1个月后研究的点燃动物中，GluR-A Flip和GluR-B Flip mRNA已恢复到对照水平。我们认为，这些变化可能导致点燃过程中齿状回谷氨酸受体敏感性增强。
