Kamphuis W, Lopes da Silva F H
Graduate School of Neuroscience, Institute of Neurobiology, University of Amsterdam, The Netherlands.
Brain Res Mol Brain Res. 1995 Mar;29(1):35-42. doi: 10.1016/0169-328x(94)00226-5.
The editing status of mRNA at the Q/R site of the glutamate receptor subtypes -A, -B, -5 and -6 modulates channel conductivity and ion selectivity of glutamate operated ion channels [4,15,26,30]. In order to investigate whether a modification of this editing process may be involved in kindling epileptogenesis, the percentage of edited variant was determined in the hippocampus of kindled rats and compared to the percentage in control animals. In the latter, GluR-A mRNA was detected only in the unedited form (with detection threshold for edited form < 0.7%), whereas GluR-B was completely edited (> 99%). For percentages were not significantly changed in Schaffer collateral/commissural pathway kindled animals that were sacrificed 24 h after the last generalized seizure. It is concluded that the increased sensitivity for the induction of seizures characteristic for Schaffer collateral kindled animals is not related to a less selective or less efficient mRNA editing process of the different glutamate receptor subunits in the hippocampus.
谷氨酸受体亚型-A、-B、-5和-6在Q/R位点的mRNA编辑状态可调节谷氨酸操纵离子通道的通道电导率和离子选择性[4,15,26,30]。为了研究这种编辑过程的改变是否可能参与点燃癫痫的发生，我们测定了点燃大鼠海马中编辑变体的百分比，并与对照动物的百分比进行比较。在对照动物中，仅检测到未编辑形式的GluR-A mRNA（编辑形式的检测阈值<0.7%），而GluR-B则完全被编辑（>99%）。在最后一次全身性发作后24小时处死的Schaffer侧支/联合通路点燃动物中，这些百分比没有显著变化。由此得出结论，Schaffer侧支点燃动物对癫痫诱导的敏感性增加与海马中不同谷氨酸受体亚基的mRNA编辑过程选择性降低或效率降低无关。
