Tatsumi Y, Taki Y, Noguchi M, Yamamoto S, Tanaka A, Shimahara Y, Ozawa K, Satouchi K, Saito K
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Nihon Geka Hokan. 1992 Sep 1;61(5):377-86.
The changes in liver mitochondrial and microsomal phospholipid molecular species were analyzed during the period of remarkably increased oxidative phosphorylation following partial hepatectomy in rabbits. At 24 hours after hepatectomy, phosphorylative activity increased significantly from 69.7 +/- 5.5 to 118.5 +/- 5.7 nmol of ATP synthesized/min/mg protein, compared to the sham operated group. The ratio of phosphatidylethanolamine to phosphatidylcholine (PE/PC) in mitochondria increased significantly in the hepatectomy group compared with the sham operated group. Remarkable changes in molecular species were observed in mitochondrial phosphatidylethanolamine. 1-Stearoyl-2-arachidonoyl species decreased in the hepatectomy group. On the other hand, microsomal phospholipids hardly changed compared with mitochondrial ones. The change in content of 1-stearoyl-2-arachidonyl phosphatidylethanolamine in mitochondria tended to return to normal levels concomitant with the normalization of phosphorylative activity. The changes in content of mitochondrial phospholipids, especially phosphatidylethanolamine, might also be related to enhancement of phosphorylative activity.
分析了兔部分肝切除术后氧化磷酸化显著增加期间肝脏线粒体和微粒体磷脂分子种类的变化。与假手术组相比，肝切除术后24小时，磷酸化活性从69.7±5.5显著增加至118.5±5.7 nmol ATP合成/分钟/毫克蛋白。肝切除组线粒体中磷脂酰乙醇胺与磷脂酰胆碱的比例（PE/PC）与假手术组相比显著增加。在线粒体磷脂酰乙醇胺中观察到分子种类的显著变化。肝切除组中1-硬脂酰-2-花生四烯酰种类减少。另一方面，与线粒体磷脂相比，微粒体磷脂几乎没有变化。线粒体中1-硬脂酰-2-花生四烯酰磷脂酰乙醇胺含量的变化随着磷酸化活性的恢复正常而趋于恢复到正常水平。线粒体磷脂含量的变化，尤其是磷脂酰乙醇胺，也可能与磷酸化活性的增强有关。
