Paprocka M, Wiedłocha A, Walzel H, Radzikowski C
Department of Tumor Immunology, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1992;40(3-4):223-7.
The A-chains of ricin obtained from Ricinus communis or mistletoe lectin I from Viscum album were coupled to the monoclonal, anti-L1210V antibody MoAb-16, using SPDP as a cross linking agent. The cytotoxic activity in vitro of these immunotoxins was compared. Each of two immunotoxins tested, applied in vitro for 1 h in appropriate doses, caused irreversible inhibition of leukemic L1210 cells proliferation. Unexpectedly, MoAb-16-MLIA immunotoxin appeared to be cytotoxic to normal bone marrow progenitor cells, as observed in NCFUS tests. Moreover, this immunotoxin revealed cytotoxic effect to the P388 leukemia cells which do not share the antigen, common within L1210 leukemia cells, detected by MoAb-16 antibody.
使用SPDP作为交联剂，将从蓖麻籽中获得的蓖麻毒素A链或来自欧洲槲寄生的槲寄生凝集素I与单克隆抗L1210V抗体MoAb - 16偶联。比较了这些免疫毒素的体外细胞毒性活性。所测试的两种免疫毒素中的每一种，以适当剂量在体外应用1小时，均导致白血病L1210细胞增殖的不可逆抑制。出乎意料的是，如在NCFUS试验中所观察到的，MoAb - 16 - MLIA免疫毒素似乎对正常骨髓祖细胞具有细胞毒性。此外，这种免疫毒素对P388白血病细胞显示出细胞毒性作用，而P388白血病细胞不具有MoAb - 16抗体所检测到的L1210白血病细胞中常见的抗原。
